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Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells
Joint Authors
Kim, Yongsoo
Chenn, Anjen
Miller, Richard J.
Szele, Francis G.
Walker, Avery S.
Goings, Gwendolyn E.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-09
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Embryonic neuroepithelia and adult subventricular zone (SVZ) stem and progenitor cells express nestin.
We characterized a transgenic line that expresses enhanced green fluorescent protein (eGFP) specified to neural tissue by the second intronic enhancer of the nestin promoter that had several novel features.
During embryogenesis, the dorsal telencephalon contained many and the ventral telencephalon few eGFP+ cells.
eGFP+ cells were found in postnatal and adult neurogenic regions.
eGFP+ cells in the SVZ expressed multiple phenotype markers, glial fibrillary acidic protein, Dlx, and neuroblast-specific molecules suggesting the transgene is expressed through the lineage.
eGFP+ cell numbers increased in the SVZ after cortical injury, suggesting this line will be useful in probing postinjury neurogenesis.
In non-neurogenic regions, eGFP was strongly expressed in oligodendrocyte progenitors, but not in astrocytes, even when they were reactive.
This eGFP+ mouse will facilitate studies of proliferative neuroepithelia and adult neurogenesis, as well as of parenchymal oligodendrocytes.
American Psychological Association (APA)
Walker, Avery S.& Goings, Gwendolyn E.& Kim, Yongsoo& Miller, Richard J.& Chenn, Anjen& Szele, Francis G.. 2011. Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells. Neural Plasticity،Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-506107
Modern Language Association (MLA)
Walker, Avery S.…[et al.]. Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells. Neural Plasticity No. 2010 (2010), pp.1-14.
https://search.emarefa.net/detail/BIM-506107
American Medical Association (AMA)
Walker, Avery S.& Goings, Gwendolyn E.& Kim, Yongsoo& Miller, Richard J.& Chenn, Anjen& Szele, Francis G.. Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells. Neural Plasticity. 2011. Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-506107
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506107