Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins
Joint Authors
Mishra, Prachi
Kale, R. K.
Kar, Anand
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-04
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Mechanism of chemoprevention by daidzein (500 μg/g bwt) was examined by injecting it subcutaneously at 16th, 18th, and 20th day postpartum, followed by counting of terminal end buds (TEBs), terminal ducts (TDs), and lobules and immunohistochemistry of ER-α, Bcl2, Bax, and caspase-3.
DNA fragmentation was also analysed to measure the apoptosis level.
Estradiol benzoate (EB) (500 ng/g bwt) and dimethyl sulphoxide (DMSO) were used as reference and vehicle, respectively.
Observations show a significant enhancement of mammary gland differentiation at postnatal day 21 (PND21) as well as PND50.
There was a significant decrease of ER-α expression at PND21 and increase in its expression at PND50, in daidzein-treated animals.
The ratio of expression of Bcl-2 to Bax proteins increased at PND50 the same whereas, it decreased at PND50 due to daidzein.
An increased expression of caspase-3 and DNA fragmentation was also seen due to daidzein at PND50.
The mammary gland of EB-treated animals showed response a somewhat similar to that of daidzein-treated animals.
American Psychological Association (APA)
Mishra, Prachi& Kar, Anand& Kale, R. K.. 2011. Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins. ISRN Oncology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-506314
Modern Language Association (MLA)
Mishra, Prachi…[et al.]. Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins. ISRN Oncology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-506314
American Medical Association (AMA)
Mishra, Prachi& Kar, Anand& Kale, R. K.. Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins. ISRN Oncology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-506314
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506314