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Identification of the DNA-Binding Domains of Human Replication Protein A That Recognize G-Quadruplex DNA
Joint Authors
Natarajan, Amarnath
Prakash, Aishwarya
Borgstahl, Gloria E. O.
Marky, Luis A.
Ouellette, Michel M.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-21
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Replication protein A (RPA), a key player in DNA metabolism, has 6 single-stranded DNA-(ssDNA-) binding domains (DBDs) A-F.
SELEX experiments with the DBDs-C, -D, and -E retrieve a 20-nt G-quadruplex forming sequence.
Binding studies show that RPA-DE binds preferentially to the G-quadruplex DNA, a unique preference not observed with other RPA constructs.
Circular dichroism experiments show that RPA-CDE-core can unfold the G-quadruplex while RPA-DE stabilizes it.
Binding studies show that RPA-C binds pyrimidine- and purine-rich sequences similarly.
This difference between RPA-C and RPA-DE binding was also indicated by the inability of RPA-CDE-core to unfold an oligonucleotide containing a TC-region 5′ to the G-quadruplex.
Molecular modeling studies of RPA-DE and telomere-binding proteins Pot1 and Stn1 reveal structural similarities between the proteins and illuminate potential DNA-binding sites for RPA-DE and Stn1.
These data indicate that DBDs of RPA have different ssDNA recognition properties.
American Psychological Association (APA)
Prakash, Aishwarya& Natarajan, Amarnath& Marky, Luis A.& Ouellette, Michel M.& Borgstahl, Gloria E. O.. 2011. Identification of the DNA-Binding Domains of Human Replication Protein A That Recognize G-Quadruplex DNA. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-506324
Modern Language Association (MLA)
Prakash, Aishwarya…[et al.]. Identification of the DNA-Binding Domains of Human Replication Protein A That Recognize G-Quadruplex DNA. Journal of Nucleic Acids No. 2011 (2011), pp.1-14.
https://search.emarefa.net/detail/BIM-506324
American Medical Association (AMA)
Prakash, Aishwarya& Natarajan, Amarnath& Marky, Luis A.& Ouellette, Michel M.& Borgstahl, Gloria E. O.. Identification of the DNA-Binding Domains of Human Replication Protein A That Recognize G-Quadruplex DNA. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-506324
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506324