Recovery of Extracellular Lipolytic Enzymes from Macrophomina phaseolina by Foam Fractionation with Air
Joint Authors
Schinke, Claudia
Germani, José Carlos
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Macrophomina phaseolina was cultivated in complex and simple media for the production of extracellular lipolytic enzymes.
Culture supernatants were batch foam fractionated for the recovery of these enzymes, and column design and operation included the use of P 2 frit (porosity 40 to 100 μm), air as sparging gas at variable flow rates, and Triton X-100 added at the beginning or gradually in aliquots.
Samples taken at intervals showed the progress of the kinetic and the efficiency parameters.
Best results were obtained with the simple medium supernatant by combining the stepwise addition of small amounts of the surfactant with the variation of the air flow rates along the separation.
Inert proteins were foamed out first, and the subsequent foamate was enriched in the enzymes, showing estimated activity recovery (R), enrichment ratio (E), and purification factor (P) of 45%, 34.7, and 2.9, respectively.
Lipases were present in the enriched foamate.
American Psychological Association (APA)
Schinke, Claudia& Germani, José Carlos. 2013. Recovery of Extracellular Lipolytic Enzymes from Macrophomina phaseolina by Foam Fractionation with Air. Enzyme Research،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-506383
Modern Language Association (MLA)
Schinke, Claudia& Germani, José Carlos. Recovery of Extracellular Lipolytic Enzymes from Macrophomina phaseolina by Foam Fractionation with Air. Enzyme Research No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-506383
American Medical Association (AMA)
Schinke, Claudia& Germani, José Carlos. Recovery of Extracellular Lipolytic Enzymes from Macrophomina phaseolina by Foam Fractionation with Air. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-506383
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506383