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Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome : The Differentiation Factors Characterization for Retinal Ganglion Cell line
Joint Authors
Yang, Ming Hui
Dibas, Adnan
Krishnamoorthy, Raghu R.
Yang, Yuan-Han
Yorio, Thomas
Chen, Wen-Cheng
Chen, Sharon Chia-Ju
Jong, Shiang-Bin
Chu, Pei-Yu
Chung, Tze Wen
Tyan, Yu Chang
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-28, 28 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-10
Country of Publication
Egypt
No. of Pages
28
Main Subjects
Abstract EN
The purpose of this paper was to characterize proteins secreted from the human nonpigmented ciliary epithelial (HNPE) cells, which have differentiated a rat retinal ganglion cell line, RGC-5.
Undifferentiated RGC-5 cells have been shown to express several marker proteins characteristic of retinal ganglion cells.
However, RGC-5 cells do not respond to N-methyl-D aspartate (NMDA), or glutamate.
HNPE cells have been shown to secrete numbers of neuropeptides or neuroproteins also found in the aqueous humor, many of which have the ability to influence the activity of neuronal cells.
This paper details the profile of HNPE cell-secreted proteins by proteomic approaches.
The experimental results revealed the identification of 132 unique proteins from the HNPE cell-conditioned SF-medium.
The biological functions of a portion of these identified proteins are involved in cell differentiation.
We hypothesized that a differentiation system of HNPE cell-conditioned SF-medium with RGC-5 cells can induce a differentiated phenotype in RGC-5 cells, with functional characteristics that more closely resemble primary cultures of rat retinal ganglion cells.
These proteins may replace harsh chemicals, which are currently used to induce cell differentiation.Erratum to “Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome: The Differentiation Factors Characterization for Retinal Ganglion Cell Line”dx.doi.org/10.1155/2011/239089
American Psychological Association (APA)
Yang, Ming Hui& Krishnamoorthy, Raghu R.& Jong, Shiang-Bin& Chu, Pei-Yu& Yang, Yuan-Han& Chen, Wen-Cheng…[et al.]. 2011. Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome : The Differentiation Factors Characterization for Retinal Ganglion Cell line. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-28.
https://search.emarefa.net/detail/BIM-506533
Modern Language Association (MLA)
Yang, Ming Hui…[et al.]. Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome : The Differentiation Factors Characterization for Retinal Ganglion Cell line. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-28.
https://search.emarefa.net/detail/BIM-506533
American Medical Association (AMA)
Yang, Ming Hui& Krishnamoorthy, Raghu R.& Jong, Shiang-Bin& Chu, Pei-Yu& Yang, Yuan-Han& Chen, Wen-Cheng…[et al.]. Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome : The Differentiation Factors Characterization for Retinal Ganglion Cell line. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-28.
https://search.emarefa.net/detail/BIM-506533
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506533