Chronic Myelogenous Leukemia Cells Contribute to the Stromal Myofibroblasts in Leukemic NODSCID Mouse In Vivo
Joint Authors
Kawasugi, Kazuo
Shirasaki, Ryosuke
Sugao, Toshihiko
Oka, Yoko
Shirafuji, Naoki
Tashiro, Haruko
Yamamoto, Tadashi
Matsuo, Takuji
Akiyama, Nobu
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We recently reported that chronic myelogenous leukemia (CML) cells converted into myofibroblasts to create a microenvironment for proliferation of CML cells in vitro.
To analyze a biological contribution of CML-derived myofibroblasts in vivo, we observed the characters of leukemic nonobese diabetes/severe combined immunodeficiency (NOD/SCID) mouse.
Bone marrow nonadherent mononuclear cells as well as human CD45-positive cells obtained from CML patients were injected to the irradiated NOD/SCID mice.
When the chimeric BCR-ABL transcript was demonstrated in blood, human CML cells were detected in NOD/SCID murine bone marrow.
And CML-derived myofibroblasts composed with the bone marrow-stroma, which produced significant amounts of human vascular endothelial growth factor A.
When the parental CML cells were cultured with myofibroblasts separated from CML cell-engrafted NOD/SCID murine bone marrow, CML cells proliferated significantly.
These observations indicate that CML cells make an adequate microenvironment for their own proliferation in vivo.
American Psychological Association (APA)
Shirasaki, Ryosuke& Tashiro, Haruko& Oka, Yoko& Matsuo, Takuji& Yamamoto, Tadashi& Sugao, Toshihiko…[et al.]. 2012. Chronic Myelogenous Leukemia Cells Contribute to the Stromal Myofibroblasts in Leukemic NODSCID Mouse In Vivo. Journal of Oncology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-506579
Modern Language Association (MLA)
Shirasaki, Ryosuke…[et al.]. Chronic Myelogenous Leukemia Cells Contribute to the Stromal Myofibroblasts in Leukemic NODSCID Mouse In Vivo. Journal of Oncology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-506579
American Medical Association (AMA)
Shirasaki, Ryosuke& Tashiro, Haruko& Oka, Yoko& Matsuo, Takuji& Yamamoto, Tadashi& Sugao, Toshihiko…[et al.]. Chronic Myelogenous Leukemia Cells Contribute to the Stromal Myofibroblasts in Leukemic NODSCID Mouse In Vivo. Journal of Oncology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-506579
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506579