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Fluorescence In Situ Hybridization for MicroRNA Detection in Archived Oral Cancer Tissues
Joint Authors
Stack, M. Sharon
Johnson, Jeffrey J.
Shi, Zonggao
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The noncoding RNA designated as microRNA (miRNA) is a large group of small single-stranded regulatory RNA and has generated wide-spread interest in human disease studies.
To facilitate delineating the role of microRNAs in cancer pathology, we sought to explore the feasibility of detecting microRNA expression in formalin-fixed paraffin-embedded (FFPE) tissues.
Using FFPE materials, we have compared fluorescent in situ hybridization (FISH) procedures to detect miR-146a with (a) different synthetic probes: regular custom DNA oligonucleotides versus locked nucleic acid (LNA) incorporated DNA oligonucleotides; (b) different reporters for the probes: biotin versus digoxigenin (DIG); (c) different visualization: traditional versus tyramide signal amplification (TSA) system; (d) different blocking reagents for endogenous peroxidase.
Finally, we performed miR-146a FISH on a commercially available oral cancer tissue microarray, which contains 40 cases of oral squamous cell carcinoma (OSCC) and 10 cases of normal epithelia from the human oral cavity.
A sample FISH protocol for detecting miR-146a is provided.
In summary, we have established reliable in situ hybridization procedures for detecting the expression of microRNA in FFPE oral cancer tissues.
This method is an important tool for studies on the involvement of microRNA in oral cancer pathology and may have potential prognostic or diagnostic value.
American Psychological Association (APA)
Shi, Zonggao& Johnson, Jeffrey J.& Stack, M. Sharon. 2012. Fluorescence In Situ Hybridization for MicroRNA Detection in Archived Oral Cancer Tissues. Journal of Oncology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-506739
Modern Language Association (MLA)
Shi, Zonggao…[et al.]. Fluorescence In Situ Hybridization for MicroRNA Detection in Archived Oral Cancer Tissues. Journal of Oncology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-506739
American Medical Association (AMA)
Shi, Zonggao& Johnson, Jeffrey J.& Stack, M. Sharon. Fluorescence In Situ Hybridization for MicroRNA Detection in Archived Oral Cancer Tissues. Journal of Oncology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-506739
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506739