![](/images/graphics-bg.png)
Induction of Antibodies and T Cell Responses by a Recombinant Influenza Virus Carrying an HIV-1 TatΔ51–59 Protein in Mice
Joint Authors
Garulli, Bruno
Ensoli, Barbara
Castrucci, Maria R.
Titti, F.
Kawaoka, Yoshihiro
Di Mario, Giuseppina
Compagnoni, D.
Stillitano, M. G.
Cafaro, A.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Recombinant influenza viruses hold promise as vectors for vaccines to prevent transmission of mucosal pathogens.
In this study, we generated a recombinant WSN/TatΔ51–59 virus in which Tat protein lacking residues 51 to 59 of the basic domain was inserted into the N-terminus of the hemagglutinin (HA) of A/WSN/33 virus.
The TatΔ51–59 insertion into the viral HA caused a 2-log reduction in viral titers in cell culture, compared with the parental A/WSN/33 virus, and severely affected virus replication in vivo.
Nevertheless, Tat-specific antibodies and T cell responses were elicited upon a single intranasal immunization of BALB/c mice with WSN/TatΔ51–59 virus.
Moreover, Tat-specific immune responses were also detected following vaccine administration via the vaginal route.
These data provide further evidence that moderately large HIV antigens can be delivered by chimeric HA constructs and elicit specific immune responses, thus increasing the options for the potential use of recombinant influenza viruses, and their derivatives, for prophylactic and therapeutic vaccines.
American Psychological Association (APA)
Garulli, Bruno& Di Mario, Giuseppina& Stillitano, M. G.& Compagnoni, D.& Titti, F.& Cafaro, A.…[et al.]. 2014. Induction of Antibodies and T Cell Responses by a Recombinant Influenza Virus Carrying an HIV-1 TatΔ51–59 Protein in Mice. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-506787
Modern Language Association (MLA)
Garulli, Bruno…[et al.]. Induction of Antibodies and T Cell Responses by a Recombinant Influenza Virus Carrying an HIV-1 TatΔ51–59 Protein in Mice. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-506787
American Medical Association (AMA)
Garulli, Bruno& Di Mario, Giuseppina& Stillitano, M. G.& Compagnoni, D.& Titti, F.& Cafaro, A.…[et al.]. Induction of Antibodies and T Cell Responses by a Recombinant Influenza Virus Carrying an HIV-1 TatΔ51–59 Protein in Mice. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-506787
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506787