Cytoskeleton Modifications and Autophagy Induction in TCam-2 Seminoma Cells Exposed to Simulated Microgravity
Joint Authors
Corano Scheri, Katia
Fabrizi, Cinzia
Fumagalli, Lorenzo
Caruso, Maria
Cammarota, Marcella
Schiraldi, Chiara
Cucina, Alessandra
Ferranti, Francesca
Masiello, Maria Grazia
Ricci, Giulia
Catizone, Angela
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-17
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The study of how mechanical forces may influence cell behavior via cytoskeleton remodeling is a relevant challenge of nowadays that may allow us to define the relationship between mechanics and biochemistry and to address the larger problem of biological complexity.
An increasing amount of literature data reported that microgravity condition alters cell architecture as a consequence of cytoskeleton structure modifications.
Herein, we are reporting the morphological, cytoskeletal, and behavioral modifications due to the exposition of a seminoma cell line (TCam-2) to simulated microgravity.
Even if no differences in cell proliferation and apoptosis were observed after 24 hours of exposure to simulated microgravity, scanning electron microscopy (SEM) analysis revealed that the change of gravity vector significantly affects TCam-2 cell surface morphological appearance.
Consistent with this observation, we found that microtubule orientation is altered by microgravity.
Moreover, the confocal analysis of actin microfilaments revealed an increase in the cell width induced by the low gravitational force.
Microtubules and microfilaments have been related to autophagy modulation and, interestingly, we found a significant autophagic induction in TCam-2 cells exposed to simulated microgravity.
This observation is of relevant interest because it shows, for the first time, TCam-2 cell autophagy as a biological response induced by a mechanical stimulus instead of a biochemical one.
American Psychological Association (APA)
Ferranti, Francesca& Caruso, Maria& Cammarota, Marcella& Masiello, Maria Grazia& Corano Scheri, Katia& Fabrizi, Cinzia…[et al.]. 2014. Cytoskeleton Modifications and Autophagy Induction in TCam-2 Seminoma Cells Exposed to Simulated Microgravity. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-506827
Modern Language Association (MLA)
Ferranti, Francesca…[et al.]. Cytoskeleton Modifications and Autophagy Induction in TCam-2 Seminoma Cells Exposed to Simulated Microgravity. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-506827
American Medical Association (AMA)
Ferranti, Francesca& Caruso, Maria& Cammarota, Marcella& Masiello, Maria Grazia& Corano Scheri, Katia& Fabrizi, Cinzia…[et al.]. Cytoskeleton Modifications and Autophagy Induction in TCam-2 Seminoma Cells Exposed to Simulated Microgravity. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-506827
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506827