Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform
Joint Authors
Bornhauser, Beat C.
Constantin, Bruno
Schaub, Marcus C.
Zuellig, Richard A.
Amstutz, Ralf
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-14
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Utrophin and dystrophin present two large proteins that link the intracellular actin cytoskeleton to the extracellular matrix via the C-terminal-associated protein complex.
Here we describe a novel short N-terminal isoform of utrophin and its protein product in various rat tissues (N-utro, 62 kDa, amino acids 1–539, comprising the actin-binding domain plus the first two spectrin repeats).
Using different N-terminal recombinant utrophin fragments, we show that actin binding exhibits pronounced negative cooperativity (affinity constants K1=∼5×106 and K2=∼1×105 M-1) and is Ca2+-insensitive.
Expression of the different fragments in COS7 cells and in myotubes indicates that the actin-binding domain alone binds exlusively to actin filaments.
The recombinant N-utro analogue binds in vitro to actin and in the cells associates to the membranes.
The results indicate that N-utro may be responsible for the anchoring of the cortical actin cytoskeleton to the membranes in muscle and other tissues.
American Psychological Association (APA)
Zuellig, Richard A.& Bornhauser, Beat C.& Amstutz, Ralf& Constantin, Bruno& Schaub, Marcus C.. 2011. Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-18.
https://search.emarefa.net/detail/BIM-506832
Modern Language Association (MLA)
Zuellig, Richard A.…[et al.]. Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-18.
https://search.emarefa.net/detail/BIM-506832
American Medical Association (AMA)
Zuellig, Richard A.& Bornhauser, Beat C.& Amstutz, Ralf& Constantin, Bruno& Schaub, Marcus C.. Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-18.
https://search.emarefa.net/detail/BIM-506832
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506832