![](/images/graphics-bg.png)
Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification : Using an HPV E6E7 mRNA Model System
Joint Authors
Johansen, Ib-Rune
Baier, Tobias
Ausen, Dag
Solli, Lars A.
Zengerle, Roland
Furuberg, Liv
O'Leary, John
Karlsen, Frank
Karlgård, Aina
Keegan, Helen
Drese, Klaus
Grønn, Petter
Falang, Inger Marie
Tofteberg, Terje R.
Gransee, Rainer
Koltay, Peter
Gulliksen, Anja
Hansen-Hagge, Thomas
Riegger, Lutz
Mielnik, Michal M.
Martin, Cara
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-22
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The paper presents the development of a “proof-of-principle” hands-free and self-contained diagnostic platform for detection of human papillomavirus (HPV) E6/E7 mRNA in clinical specimens.
The automated platform performs chip-based sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection with minimal user interfacing.
It consists of two modular prototypes, one for sample preparation and one for amplification and detection; however, a common interface is available to facilitate later integration into one single module.
Nucleic acid extracts (n=28) from cervical cytology specimens extracted on the sample preparation chip were tested using the PreTect HPV-Proofer and achieved an overall detection rate for HPV across all dilutions of 50%–85.7%.
A subset of 6 clinical samples extracted on the sample preparation chip module was chosen for complete validation on the NASBA chip module.
For 4 of the samples, a 100% amplification for HPV 16 or 33 was obtained at the 1 : 10 dilution for microfluidic channels that filled correctly.
The modules of a “sample-in, answer-out” diagnostic platform have been demonstrated from clinical sample input through sample preparation, amplification and final detection.
American Psychological Association (APA)
Gulliksen, Anja& Keegan, Helen& Martin, Cara& O'Leary, John& Solli, Lars A.& Falang, Inger Marie…[et al.]. 2011. Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification : Using an HPV E6E7 mRNA Model System. Journal of Oncology،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-506884
Modern Language Association (MLA)
Gulliksen, Anja…[et al.]. Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification : Using an HPV E6E7 mRNA Model System. Journal of Oncology No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-506884
American Medical Association (AMA)
Gulliksen, Anja& Keegan, Helen& Martin, Cara& O'Leary, John& Solli, Lars A.& Falang, Inger Marie…[et al.]. Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification : Using an HPV E6E7 mRNA Model System. Journal of Oncology. 2011. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-506884
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506884