High Throughput Quantitative Bioanalytical LCMSMS Determination of Gemifloxacin in Human Urine
Joint Authors
Abdelhameed, Ali Saber
Angawi, Rihab F.
Darwish, Hany W.
Kadi, Adnan A.
Attwa, Mohamed W.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A highly specific, sensitive, and rapid method, to quantify gemifloxacin in human urine using HPLC coupled to the triple quadrupole mass spectrometer system, was developed and validated.
Gemifloxacin and ofloxacin (internal standard) were rapidly extracted from urine samples without any tedious pretreatment procedure.
Urine samples were filtered through a Millex-GP, 0.22 μm syringe filter.
Optimal chromatographic separation of the analytes was achieved on Zorbax SB-C18 (30 mm × 2 mm i.d., 3.5 μm maintained at ambient temperature).
The mobile phase consisted of 0.1% formic acid (pH 3.2) and acetonitrile (80 : 20) and a flow rate of 0.2 mL min−1 for 4 min.
The analytes were monitored by electrospray ionization in positive ion multiple reaction monitoring mode.
The method provided a linear response (r=0.9998) from a quantitation range of 5 ng mL−1 to at least 500 ng mL−1.
The mean extraction recovery % of gemifloxacin from spiked human urine was 101.33 ± 2.58%.
The reproducibility of the method was reliable with the intra- and inter-day precision of <2% and accuracy within 2%.
The established method was reliably applied for the determination of gemifloxacin in volunteers’ urine samples with the mean recoveries of gemifloxacin from Factive tablets 320 mg > 97.0%.
American Psychological Association (APA)
Kadi, Adnan A.& Angawi, Rihab F.& Attwa, Mohamed W.& Darwish, Hany W.& Abdelhameed, Ali Saber. 2013. High Throughput Quantitative Bioanalytical LCMSMS Determination of Gemifloxacin in Human Urine. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-506939
Modern Language Association (MLA)
Kadi, Adnan A.…[et al.]. High Throughput Quantitative Bioanalytical LCMSMS Determination of Gemifloxacin in Human Urine. Journal of Chemistry No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-506939
American Medical Association (AMA)
Kadi, Adnan A.& Angawi, Rihab F.& Attwa, Mohamed W.& Darwish, Hany W.& Abdelhameed, Ali Saber. High Throughput Quantitative Bioanalytical LCMSMS Determination of Gemifloxacin in Human Urine. Journal of Chemistry. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-506939
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506939