Spider Silk as Guiding Biomaterial for Human Model Neurons
Joint Authors
Bicker, Gerd
Radtke, Christine
Strauß, Sarah
Roloff, Frank
Vogt, Peter M.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Over the last years, a number of therapeutic strategies have emerged to promote axonal regeneration.
An attractive strategy is the implantation of biodegradable and nonimmunogenic artificial scaffolds into injured peripheral nerves.
In previous studies, transplantation of decellularized veins filled with spider silk for bridging critical size nerve defects resulted in axonal regeneration and remyelination by invading endogenous Schwann cells.
Detailed interaction of elongating neurons and the spider silk as guidance material is unknown.
To visualize direct cellular interactions between spider silk and neurons in vitro, we developed an in vitro crossed silk fiber array.
Here, we describe in detail for the first time that human (NT2) model neurons attach to silk scaffolds.
Extending neurites can bridge gaps between single silk fibers and elongate afterwards on the neighboring fiber.
Culturing human neurons on the silk arrays led to an increasing migration and adhesion of neuronal cell bodies to the spider silk fibers.
Within three to four weeks, clustered somata and extending neurites formed ganglion-like cell structures.
Microscopic imaging of human neurons on the crossed fiber arrays in vitro will allow for a more efficient development of methods to maximize cell adhesion and neurite growth on spider silk prior to transplantation studies.
American Psychological Association (APA)
Roloff, Frank& Strauß, Sarah& Vogt, Peter M.& Bicker, Gerd& Radtke, Christine. 2014. Spider Silk as Guiding Biomaterial for Human Model Neurons. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-507049
Modern Language Association (MLA)
Roloff, Frank…[et al.]. Spider Silk as Guiding Biomaterial for Human Model Neurons. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-507049
American Medical Association (AMA)
Roloff, Frank& Strauß, Sarah& Vogt, Peter M.& Bicker, Gerd& Radtke, Christine. Spider Silk as Guiding Biomaterial for Human Model Neurons. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-507049
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507049