Technical Challenges in the Derivation of Human Pluripotent Cells
Joint Authors
Noisa, Parinya
Parnpai, Rangsun
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
It has long been discovered that human pluripotent cells could be isolated from the blastocyst state of embryos and called human embryonic stem cells (ESCs).
These cells can be adapted and propagated indefinitely in culture in an undifferentiated manner as well as differentiated into cell representing the three major germ layers: endoderm, mesoderm, and ectoderm.
However, the derivation of human pluripotent cells from donated embryos is limited and restricted by ethical concerns.
Therefore, various approaches have been explored and proved their success.
Human pluripotent cells can also be derived experimentally by the nuclear reprogramming of somatic cells.
These techniques include somatic cell nuclear transfer (SCNT), cell fusion and overexpression of pluripotent genes.
In this paper, we discuss the technical challenges of these approaches for nuclear reprogramming, involving their advantages and limitations.
We will also highlight the possible applications of these techniques in the study of stem cell biology.
American Psychological Association (APA)
Noisa, Parinya& Parnpai, Rangsun. 2011. Technical Challenges in the Derivation of Human Pluripotent Cells. Stem Cells International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-507176
Modern Language Association (MLA)
Noisa, Parinya& Parnpai, Rangsun. Technical Challenges in the Derivation of Human Pluripotent Cells. Stem Cells International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-507176
American Medical Association (AMA)
Noisa, Parinya& Parnpai, Rangsun. Technical Challenges in the Derivation of Human Pluripotent Cells. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-507176
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507176