![](/images/graphics-bg.png)
Comparison of Behaviour in Different Liquids and in Cells of Gold Nanorods and Spherical Nanoparticles Modified by Linear Polyethyleneimine and Bovine Serum Albumin
Joint Authors
Poletaeva, Julia E.
Pyshnaya, Inna A.
Razum, Kristina V.
Pyshnyi, Dmitrii V.
Ryabchikova, Elena I.
Zenkova, Marina A.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-01
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Gold nanorods (GNRs) are considered one of the most promising forms of nanoparticles for nanobiotechnology; however, the problem of their toxicity is currently not resolved.
We synthesised GNRs, modified with linear polyethyleneimine (PEI-GNRs), and examined their physicochemical and some biological properties in comparison with GNRs modified with BSA and spherical gold nanoparticles (sGNPs) modified with the same agents.
The influence of the buffer, cell culture media, and serum on hydrodynamic diameter and zeta potential of all GNPs was studied.
Simultaneously, the size, shape, and formation of a corona were examined by transmission electron microscopy (TEM).
PEI-GNRs and GNPs were nontoxic for BHK-21 and HeLa cells (MTT test).
Penetration of all GNPs into BHK-21, melanoma B16, and HeLa cells was examined after 30 min, 3 h, and 24 h of incubation using TEM ultrathin sections.
PEI-GNRs and PEI-sGNPs demonstrated fast and active penetration into cells by caveolin-dependent and lipid raft-mediated endocytosis and accumulated in endosomes and lysosomes.
BSA-modified GNPs showed prolonged flotation and a significant delay in cell penetration.
The results show that the charge of initial NPs determines penetration into cells.
Thus, the designed PEI-GNRs were nontoxic and stable in cell culture media and could efficiently penetrate cells.
American Psychological Association (APA)
Pyshnaya, Inna A.& Razum, Kristina V.& Poletaeva, Julia E.& Pyshnyi, Dmitrii V.& Zenkova, Marina A.& Ryabchikova, Elena I.. 2014. Comparison of Behaviour in Different Liquids and in Cells of Gold Nanorods and Spherical Nanoparticles Modified by Linear Polyethyleneimine and Bovine Serum Albumin. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-507203
Modern Language Association (MLA)
Pyshnaya, Inna A.…[et al.]. Comparison of Behaviour in Different Liquids and in Cells of Gold Nanorods and Spherical Nanoparticles Modified by Linear Polyethyleneimine and Bovine Serum Albumin. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-507203
American Medical Association (AMA)
Pyshnaya, Inna A.& Razum, Kristina V.& Poletaeva, Julia E.& Pyshnyi, Dmitrii V.& Zenkova, Marina A.& Ryabchikova, Elena I.. Comparison of Behaviour in Different Liquids and in Cells of Gold Nanorods and Spherical Nanoparticles Modified by Linear Polyethyleneimine and Bovine Serum Albumin. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-507203
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507203