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Interfacing Sca-1pos Mesenchymal Stem Cells with Biocompatible Scaffolds with Different Chemical Composition and Geometry
Joint Authors
Bonmassar, E.
Di Francesco, A. M.
Minieri, M.
Di Nardo, P.
Laudisi, A.
Franzese, O.
Pagliari, F.
Forte, G.
Fiaccavento, R.
Cossa, P.
Pagliari, S.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-07-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
An immortalized murine mesenchymal stem cell line (mTERT-MSC) enriched for Linneg/Sca-1pos fraction has been obtained through the transfection of MSC with murine TERT and single-cell isolation.
Such cell line maintained the typical MSC self-renewal capacity and continuously expressed MSC phenotype.
Moreover, mTERT-MSC retained the functional features of freshly isolated MSC in culture without evidence of senescence or spontaneous differentiation events.
Thus, mTERT-MSC have been cultured onto PLA films, 30 and 100 μm PLA microbeads, and onto unpressed and pressed HYAFF-11 scaffolds.
While the cells adhered preserving their morphology on PLA films, clusters of mTERT-MSC were detected on PLA beads and unpressed fibrous scaffolds.
Finally, mTERT-MSC were not able to colonize the inner layers of pressed HYAFF-11.
Nevertheless, such cell line displayed the ability to preserve Sca-1 expression and to retain multilineage potential when appropriately stimulated on all the scaffolds tested.
American Psychological Association (APA)
Forte, G.& Franzese, O.& Pagliari, S.& Pagliari, F.& Di Francesco, A. M.& Cossa, P.…[et al.]. 2009. Interfacing Sca-1pos Mesenchymal Stem Cells with Biocompatible Scaffolds with Different Chemical Composition and Geometry. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-507417
Modern Language Association (MLA)
Forte, G.…[et al.]. Interfacing Sca-1pos Mesenchymal Stem Cells with Biocompatible Scaffolds with Different Chemical Composition and Geometry. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-507417
American Medical Association (AMA)
Forte, G.& Franzese, O.& Pagliari, S.& Pagliari, F.& Di Francesco, A. M.& Cossa, P.…[et al.]. Interfacing Sca-1pos Mesenchymal Stem Cells with Biocompatible Scaffolds with Different Chemical Composition and Geometry. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-507417
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507417