Potassium Current Is Not Affected by Long-Term Exposure to Ghrelin or GHRP-6 in Somatotropes GC Cells
Joint Authors
Cervantes Acosta, Patricia
Hernández Beltran, Antonio
Flores-Hernández, Jorge L. V.
Domínguez Mancera, Belisario
Barrientos Morales, Manuel
Martínez Hernandez, José M.
Monjaraz Guzman, Eduardo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-24
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Ghrelin is a growth hormone (GH) secretagogue (GHS) and GHRP-6 is a synthetic peptide analogue; both act through the GHS receptor.
GH secretion depends directly on the intracellular concentration of Ca2+; this is determined from the intracellular reserves and by the entrance of Ca2+ through the voltage-dependent calcium channels, which are activated by the membrane depolarization.
Membrane potential is mainly determined by K+ channels.
In the present work, we investigated the effect of ghrelin (10 nM) or GHRP-6 (100 nM) for 96 h on functional expression of voltage-dependent K+ channels in rat somatotropes: GC cell line.
Physiological patch-clamp whole-cell recording was used to register the K+ currents.
With Cd2+ (1 mM) and tetrodotoxin (1 μm) in the bath solution recording, three types of currents were characterized on the basis of their biophysical and pharmacological properties.
GC cells showed a K+ current with a transitory component (IA) sensitive to 4-aminopyridine, which represents ~40% of the total outgoing current; a sustained component named delayed rectifier (IK), sensitive to tetraethylammonium; and a third type of K+ current was recorded at potentials more negative than −80 mV, permitting the entrance of K+ named inward rectifier (KIR).
Chronic treatment with ghrelin or GHRP-6 did not modify the functional expression of K+ channels, without significant changes (P<0.05) in the amplitudes of the three currents observed; in addition, there were no modifications in their biophysical properties and kinetic activation or inactivation.
American Psychological Association (APA)
Domínguez Mancera, Belisario& Monjaraz Guzman, Eduardo& Flores-Hernández, Jorge L. V.& Barrientos Morales, Manuel& Martínez Hernandez, José M.& Hernández Beltran, Antonio…[et al.]. 2013. Potassium Current Is Not Affected by Long-Term Exposure to Ghrelin or GHRP-6 in Somatotropes GC Cells. Journal of Biophysics،Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-507619
Modern Language Association (MLA)
Domínguez Mancera, Belisario…[et al.]. Potassium Current Is Not Affected by Long-Term Exposure to Ghrelin or GHRP-6 in Somatotropes GC Cells. Journal of Biophysics No. 2013 (2013), pp.1-16.
https://search.emarefa.net/detail/BIM-507619
American Medical Association (AMA)
Domínguez Mancera, Belisario& Monjaraz Guzman, Eduardo& Flores-Hernández, Jorge L. V.& Barrientos Morales, Manuel& Martínez Hernandez, José M.& Hernández Beltran, Antonio…[et al.]. Potassium Current Is Not Affected by Long-Term Exposure to Ghrelin or GHRP-6 in Somatotropes GC Cells. Journal of Biophysics. 2013. Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-507619
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507619