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Curdione Plays an Important Role in the Inhibitory Effect of Curcuma aromatica on CYP3A4 in Caco-2 Cells
Joint Authors
Takatani-Nakase, Tomoka
Takahashi, Kyoko
Tanaka, Ken
Kuo, Tsu-Hsiang
Komatsu, Katsuko
Takahashi, Koichi
Hayashi-Nakamura, Emi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Curcuma aromatica is a plant belonging to genus Curcuma of family Zingiberaceae and is widely used as supplements in Japan.
Rhizomes of C.
aromatica have curcumin as a major yellow pigment and curdione as a main ingredient of essential oils.
In this study, we investigated the affect of C.
aromatica on CYP3A4 using 1α,25-(OH)2-D3-treated Caco-2 clone cells.
Caco-2 cells were treated with methanol extract (0.1 mg ml−1), its hexane soluble fraction (0.1 mg ml−1), curcumin (4 μM) and curdione (20 μM) for 72 hours.
Nifedipine was used as a substrate of CYP3A4.
Methanol extract, hexane fraction and curdione inhibited the formation of oxidized nifedipine by 50–70%, and curcumin showed no effect.
The IC50s of methanol extract, hexane fraction and curdione to oxidized nifedipine formation were 21, 14 and 3.9 μg ml−1 (16.9 μM), respectively.
The content of curdione in methanol extract was 11.4%.
Moreover, all of methanol extract, hexane fraction and curdione decreased CYP3A4 protein expression but had no affect on CYP3A4 mRNA expression.
Our results showed that these drugs further decreased the CYP3A4 protein expression level after the protein synthesis was inhibited by cychroheximide.
These findings suggest that curdione plays an important role in the CYP3A4 inhibitory activity of C.
aromatica and curdione might inhibit the activity by accelerating the degradation of CYP3A4.
American Psychological Association (APA)
Kuo, Tsu-Hsiang& Hayashi-Nakamura, Emi& Takatani-Nakase, Tomoka& Tanaka, Ken& Takahashi, Kyoko& Komatsu, Katsuko…[et al.]. 2011. Curdione Plays an Important Role in the Inhibitory Effect of Curcuma aromatica on CYP3A4 in Caco-2 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-507631
Modern Language Association (MLA)
Kuo, Tsu-Hsiang…[et al.]. Curdione Plays an Important Role in the Inhibitory Effect of Curcuma aromatica on CYP3A4 in Caco-2 Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-507631
American Medical Association (AMA)
Kuo, Tsu-Hsiang& Hayashi-Nakamura, Emi& Takatani-Nakase, Tomoka& Tanaka, Ken& Takahashi, Kyoko& Komatsu, Katsuko…[et al.]. Curdione Plays an Important Role in the Inhibitory Effect of Curcuma aromatica on CYP3A4 in Caco-2 Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-507631
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507631