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Effect of High-Fat Diet upon Inflammatory Markers and Aortic Stiffening in Mice
Joint Authors
Borelli, Primavera
Santana, Andre Bento Chaves
Santos, Ed Wilson Cavalcante Oliveira
Silva, Juliane Cristina S.
Krieger, José Eduardo
Alves, Tatiana P.
Souza Oliveira, Thais Cristina de
Bianconi, Barbara Lobo
Barauna, Valerio Garrone
Irigoyen, Maria Claudia Costa
Lacchini, Silvia
Fiorino, Patricia
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Changes in lifestyle such as increase in high-fat food consumption are an important cause for vascular diseases.
The present study aimed to investigate the involvement of ACE and TGF-β in the aorta stiffness induced by high-fat diet.
C57BL/6 male mice were divided in two groups according to their diet for 8 weeks: standard diet (ST) and high-fat diet (HF).
At the end of the protocol, body weight gain, adipose tissue content, serum lipids and glucose levels, and aorta morphometric and biochemical measurements were performed.
Analysis of collagen fibers by picrosirius staining of aorta slices showed that HF diet promoted increase of thin (55%) and thick (100%) collagen fibers deposition and concomitant disorganization of these fibers orientations in the aorta vascular wall (50%).
To unravel the mechanism involved, myeloperoxidase (MPO) and angiotensin I converting enzyme (ACE) were evaluated by protein expression and enzyme activity.
HF diet increased MPO (90%) and ACE (28%) activities, as well as protein expression of ACE.
TGF-β was also increased in aorta tissue of HF diet mice after 8 weeks.
Altogether, we have observed that the HF diet-induced aortic stiffening may be associated with increased oxidative stress damage and activation of the RAS in vascular tissue.
American Psychological Association (APA)
Santana, Andre Bento Chaves& Souza Oliveira, Thais Cristina de& Bianconi, Barbara Lobo& Barauna, Valerio Garrone& Santos, Ed Wilson Cavalcante Oliveira& Alves, Tatiana P.…[et al.]. 2014. Effect of High-Fat Diet upon Inflammatory Markers and Aortic Stiffening in Mice. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-507644
Modern Language Association (MLA)
Santana, Andre Bento Chaves…[et al.]. Effect of High-Fat Diet upon Inflammatory Markers and Aortic Stiffening in Mice. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-507644
American Medical Association (AMA)
Santana, Andre Bento Chaves& Souza Oliveira, Thais Cristina de& Bianconi, Barbara Lobo& Barauna, Valerio Garrone& Santos, Ed Wilson Cavalcante Oliveira& Alves, Tatiana P.…[et al.]. Effect of High-Fat Diet upon Inflammatory Markers and Aortic Stiffening in Mice. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-507644
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507644