San-Huang-Xie-Xin-Tang Prevents Rat Hearts from IschemiaReperfusion-Induced Apoptosis through eNOS and MAPK Pathways
Joint Authors
Ke, Hung-Jen
Liou, Shu-Fen
Yeh, Jwu-Lai
Lin, Hung-Hong
Hsu, Jong-Hau
Chen, Ing-Jun
Liang, Jyh-Chong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
San-Huang-Xie-Xin-Tang (SHXT) is a traditional Chinese medication consisting of three herbs, namely Coptidis rhizome, Scutellariae radix and Rhei rhizome.
This study aimed to examine the cardioprotective effects of SHXT in a rat model of acute myocardial apoptosis induced by ischemia/reperfusion (I/R).
Vehicle (intravenous saline) or SHXT (intravenous or oral) was administered prior to I/R (occlusion of left coronary artery for 45 min followed by reperfusion for 2 h).
In the vehicle group, myocardial I/R caused myocardial infarction with increased plasma cardiac enzymes, severe arrhythmia and mortality.
Myocardial apoptosis was induced by I/R as evidenced by DNA ladder and Bcl-2/Bax ratio.
In the SHXT group, we found that SHXT significantly reduced plasma levels of cardiac enzymes, arrhythmia scores (from 5 ± 1 to 2 ± 1, P<.01) and mortality rate (from 53 to 0%, P<.01).
In addition, pretreatment with intravenous SHXT reduced the infarct size dose-dependently when compared with the vehicle group (10 mg kg−1: 14.0 ± 0.2 versus 44.5 ± 5.0%, and 30 mg kg−1: 6.2 ± 1.2% versus 44.5 ± 5.0%, both P<.01).
Similarly, oral administration of SHXT reduced the infarct size dose-dependently.
Furthermore, SHXT markedly decreased the apoptosis induced by I/R with increased Bcl-2/Bax ratio.
Finally, we found that SHXT counteracted the I/R-induced downstream signaling, resulting in increased myocardial eNOS expression and plasma nitrite, and decreased activation of ERK1/2, p38 and JNK.
These data suggest that SHXT has cardioprotective effects against I/R-induced apoptosis, and that these effects are mediated, at least in part, by eNOS and MAPK pathways.
American Psychological Association (APA)
Liou, Shu-Fen& Ke, Hung-Jen& Hsu, Jong-Hau& Liang, Jyh-Chong& Lin, Hung-Hong& Chen, Ing-Jun…[et al.]. 2011. San-Huang-Xie-Xin-Tang Prevents Rat Hearts from IschemiaReperfusion-Induced Apoptosis through eNOS and MAPK Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-507725
Modern Language Association (MLA)
Liou, Shu-Fen…[et al.]. San-Huang-Xie-Xin-Tang Prevents Rat Hearts from IschemiaReperfusion-Induced Apoptosis through eNOS and MAPK Pathways. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-507725
American Medical Association (AMA)
Liou, Shu-Fen& Ke, Hung-Jen& Hsu, Jong-Hau& Liang, Jyh-Chong& Lin, Hung-Hong& Chen, Ing-Jun…[et al.]. San-Huang-Xie-Xin-Tang Prevents Rat Hearts from IschemiaReperfusion-Induced Apoptosis through eNOS and MAPK Pathways. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-507725
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507725