![](/images/graphics-bg.png)
Perfusion Flow Enhances Osteogenic Gene Expression and the Infiltration of Osteoblasts and Endothelial Cells into Three-Dimensional Calcium Phosphate Scaffolds
Joint Authors
Tsai, Chung-Jui
Donahue, Seth W.
Goldman, Jeremy
Barron, Matthew J.
Source
International Journal of Biomaterials
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Maintaining cellular viability in vivo and in vitro is a critical issue in three-dimensional bone tissue engineering.
While the use of osteoblast/endothelial cell cocultures on three-dimensional constructs has shown promise for increasing in vivo vascularization, in vitro maintenance of cellular viability remains problematic.
This study used perfusion flow to increase osteogenic and angiogenic gene expression, decrease hypoxic gene expression, and increase cell and matrix coverage in osteoblast/endothelial cell co-cultures.
Mouse osteoblast-like cells (MC3T3-E1) were cultured alone and in co-culture with mouse microvascular endothelial cells (EOMA) on three-dimensional scaffolds for 1, 2, 7, and 14 days with or without perfusion flow.
mRNA levels were determined for several osteogenic, angiogenic, and hypoxia-related genes, and histological analysis was performed.
Perfusion flow downregulated hypoxia-related genes (HIF-1α, VEGF, and OPN) at early timepoints, upregulated osteogenic genes (ALP and OCN) at 7 days, and downregulated RUNX-2 and VEGF mRNA at 14 days in osteoblast monocultures.
Perfusion flow increased cell number, coverage of the scaffold perimeter, and matrix area in the center of scaffolds at 14 days.
Additionally, perfusion flow increased the length of endothelial cell aggregations within co-cultures.
These suggest perfusion stimulated co-cultures provide a means of increasing osteogenic and angiogenic activity.
American Psychological Association (APA)
Barron, Matthew J.& Goldman, Jeremy& Tsai, Chung-Jui& Donahue, Seth W.. 2012. Perfusion Flow Enhances Osteogenic Gene Expression and the Infiltration of Osteoblasts and Endothelial Cells into Three-Dimensional Calcium Phosphate Scaffolds. International Journal of Biomaterials،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-507784
Modern Language Association (MLA)
Barron, Matthew J.…[et al.]. Perfusion Flow Enhances Osteogenic Gene Expression and the Infiltration of Osteoblasts and Endothelial Cells into Three-Dimensional Calcium Phosphate Scaffolds. International Journal of Biomaterials No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-507784
American Medical Association (AMA)
Barron, Matthew J.& Goldman, Jeremy& Tsai, Chung-Jui& Donahue, Seth W.. Perfusion Flow Enhances Osteogenic Gene Expression and the Infiltration of Osteoblasts and Endothelial Cells into Three-Dimensional Calcium Phosphate Scaffolds. International Journal of Biomaterials. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-507784
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507784