Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC
Joint Authors
Srivastava, Sharad Kr.
Govindarajan, Raghavan
Rawat, A. K. S.
Pandey, Madan Mohan
Singh, D. P.
Singh, A. P.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-28
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Heracleum candicans Wall.
ex DC.
(Apiaceae) is widely used in Indian system of medicines as an aphrodisiac, nerve tonic and also in the treatment of skin diseases with reports of being rich in furocoumarins which are extensively used in pharmaceutical industry for their photosensitizing activity on human skin.
A simple high performance thin layer chromatography (HPTLC) method has been developed for the simultaneous determination of psoralen (1) and heraclenol (2) in the fruits of H.
candicans.
The analytes were separated on silica gel F254 plates with toluene : diethy lether : acetic acid (6 : 4 : 1) and scanned densitometrically at 350 nm.
The method was validated in terms of precision, repeatability, and accuracy.
The linear range for psoralen and heraclenol was found to be 1–5 μg spot−1 and 1–4 μg spot−1 with correlation coefficient of 0.973 and 0.964, respectively.
The two compounds were quantified in fruits of H.
candicans and were found to be present in the range of 0.021–0.036% and 0.029–0.043% w/w.
The method was found to be very simple, accurate, precise, and economical and can be used for routine quality control.
American Psychological Association (APA)
Rawat, A. K. S.& Singh, A. P.& Singh, D. P.& Pandey, Madan Mohan& Govindarajan, Raghavan& Srivastava, Sharad Kr.. 2013. Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-507798
Modern Language Association (MLA)
Rawat, A. K. S.…[et al.]. Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC. Journal of Chemistry No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-507798
American Medical Association (AMA)
Rawat, A. K. S.& Singh, A. P.& Singh, D. P.& Pandey, Madan Mohan& Govindarajan, Raghavan& Srivastava, Sharad Kr.. Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC. Journal of Chemistry. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-507798
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507798