A Method for the In Vivo Measurement of Zebrafish Tissue Neutrophil Lifespan
Joint Authors
Dixon, Giles
Whyte, Moira K. B.
Loynes, Catherine A.
Elks, Philip M.
Renshaw, Stephen A.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-16
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Neutrophil function is thought to be regulated, in large part, by limitation of lifespan by apoptosis.
A number of studies suggest that circulating neutrophils have a half-life of approximately 6 hours, although contradictory evidence exists.
Measuring tissue neutrophil lifespan, however, is more problematic.
It is thought that tissue neutrophils survive longer, perhaps with a half-life in the order of 3–5 days, but this has never been directly measured.
Zebrafish are an emerging model organism, with several advantages for the study of vertebrate immunity.
In zebrafish, neutrophils constitutively assume tissue locations allowing their direct study in vivo.
Using a transgenic approach, neutrophils were labelled with a photoconvertible pigment, Kaede.
Photoconversion parameters were optimised and the stability of the Kaede confirmed.
Individual neutrophils were photoconverted by scanning a confocal 405 nm laser specifically over each cell and their survival monitored for 48 hours, revealing an in vivo half-life for zebrafish tissue neutrophils of around 120 hours (117.7 hrs, 95% CI 95.67–157.8).
Laser energy did not extend neutrophil lifespan, and we conclude that this represents a lower bound for the lifespan of a resting tissue neutrophil in the developing zebrafish larva.
This is the first direct measurement of the lifespan of an in vivo tissue neutrophil.
American Psychological Association (APA)
Dixon, Giles& Elks, Philip M.& Loynes, Catherine A.& Whyte, Moira K. B.& Renshaw, Stephen A.. 2012. A Method for the In Vivo Measurement of Zebrafish Tissue Neutrophil Lifespan. ISRN Hematology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-507804
Modern Language Association (MLA)
Dixon, Giles…[et al.]. A Method for the In Vivo Measurement of Zebrafish Tissue Neutrophil Lifespan. ISRN Hematology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-507804
American Medical Association (AMA)
Dixon, Giles& Elks, Philip M.& Loynes, Catherine A.& Whyte, Moira K. B.& Renshaw, Stephen A.. A Method for the In Vivo Measurement of Zebrafish Tissue Neutrophil Lifespan. ISRN Hematology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-507804
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507804