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Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks
Joint Authors
Akiba, Yuko
Tsunomori, Yoshie
Katsube, Kazunori
Atsuta, Junko
Kishi, Ryoko
Fukushima, Hiroshi
Source
International Journal of Microbiology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-06-24
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
A set of four duplex SYBR Green I PCR (SG-PCR) assay combined with DNA extraction using QIAamp DNA Stool Mini kit was evaluated for the detection of foodborne bacteria from 21 foodborne outbreaks.
The causative pathogens were detected in almost all cases in 2 hours or less.
The first run was for the detection of 8 main foodborne pathogens in 5 stool specimens within 2 hours and the second run was for the detection of other unusual suspect pathogens within a further 45 minutes.
After 2 to 4 days, the causative agents were isolated and identified.
The results proved that for comprehensive and rapid molecular diagnosis in foodborne outbreaks, Duplex SG-PCR assay is not only very useful, but is also economically viable for one-step differentiation of causative pathogens in fecal specimens obtained from symptomatic patients.
This then allows for effective diagnosis and management of foodborne outbreaks.
American Psychological Association (APA)
Fukushima, Hiroshi& Katsube, Kazunori& Tsunomori, Yoshie& Kishi, Ryoko& Atsuta, Junko& Akiba, Yuko. 2009. Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks. International Journal of Microbiology،Vol. 2009, no. 2009, pp.1-13.
https://search.emarefa.net/detail/BIM-507975
Modern Language Association (MLA)
Fukushima, Hiroshi…[et al.]. Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks. International Journal of Microbiology No. 2009 (2009), pp.1-13.
https://search.emarefa.net/detail/BIM-507975
American Medical Association (AMA)
Fukushima, Hiroshi& Katsube, Kazunori& Tsunomori, Yoshie& Kishi, Ryoko& Atsuta, Junko& Akiba, Yuko. Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks. International Journal of Microbiology. 2009. Vol. 2009, no. 2009, pp.1-13.
https://search.emarefa.net/detail/BIM-507975
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507975