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Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding : Influence of Membrane Components
Joint Authors
Simakova, Olga
Arispe, Nelson J.
Source
International Journal of Alzheimer's Disease
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
We performed a fluorescent analysis of the binding of Aβ to the surface membrane of different types of cells lines such as PC12, GT1-7, and ex vivo neurons.
Analyses were performed on sorted cells with membrane bound Aβ Competitive binding between Aβ phosphatidyl serine- (PtdSer-) specific binder annexin V and an anti-PtdSer antibody provided compelling data confirming the involvement of PtdSer as one of the surface membrane signal molecules for Aβ.
We found that populations of cells that exhibited high surface membrane binding affinity for Aβ also show higher membrane cholesterol levels compared to cells that did not bind Aβ.
This direct relationship was upheld in cholesterol-enriched or cholesterol-depleted cell membranes.
We conclude that the initial process for the cell-selective binding by Aβ, to later conversion of elemental Aβ units into larger structures such as fibrils or to the potentially toxic ion channel aggregates, is highly influenced by the membrane content of PtdSer and cholesterol in the cell surface membrane.
American Psychological Association (APA)
Simakova, Olga& Arispe, Nelson J.. 2011. Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding : Influence of Membrane Components. International Journal of Alzheimer's Disease،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-507977
Modern Language Association (MLA)
Simakova, Olga& Arispe, Nelson J.. Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding : Influence of Membrane Components. International Journal of Alzheimer's Disease No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-507977
American Medical Association (AMA)
Simakova, Olga& Arispe, Nelson J.. Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding : Influence of Membrane Components. International Journal of Alzheimer's Disease. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-507977
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507977