The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response
Joint Authors
Tchatalbachev, Svetlin
Domann, Eugen
Quintanar Haro, Orlando Daniel
Chakraborty, Trinad
Kraemer, Thomas Daniel
Source
International Journal of Microbiology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Based on Toll/interleukin-1 receptor (TIR) domain structure homology, we detected a previously uncharacterized gene encoding for a TIR domain containing protein (Tcp) in the genome of Enterococcus faecalis.
We assigned this gene the name tcpF (as in Tcp of E.
faecalis).
Screening of E.
faecalis samples revealed that tcpF is more common in isolates from urinary tract infections (UTIs) than in human faecal flora.
tcpF alleles showed moderate single nucleotide polymorphism (SNP) among UTI isolates.
Infection of mouse RAW264.7 macrophages with a tcpF knock-out mutant led to elevated cytokine response compared to the isogenic wild type E.
faecalis strain.
In silico analysis predicted significant tertiary structure homology to the TIR domain of human TLR1 (TLR1-TIR).
When transiently expressed in cultured eukaryotic cells, TcpF caused suppression of TLR2-dependent NF-κB activation suggesting for TcpF a role as a factor in E.
faecalis that benefits colonization by modulating the host’s immune responses.
American Psychological Association (APA)
Kraemer, Thomas Daniel& Quintanar Haro, Orlando Daniel& Domann, Eugen& Chakraborty, Trinad& Tchatalbachev, Svetlin. 2014. The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response. International Journal of Microbiology،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-508034
Modern Language Association (MLA)
Kraemer, Thomas Daniel…[et al.]. The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response. International Journal of Microbiology No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-508034
American Medical Association (AMA)
Kraemer, Thomas Daniel& Quintanar Haro, Orlando Daniel& Domann, Eugen& Chakraborty, Trinad& Tchatalbachev, Svetlin. The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response. International Journal of Microbiology. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-508034
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508034