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Different Effects of Mg2+ and Zn2+ on the Two Sites for Alkylammonium Compounds in Pseudomonas aeruginosa Phosphorylcholine Phosphatase
Joint Authors
Lisa, Angela Teresita
Boetsch, Cristhian
Domenech, Carlos Eduardo
Otero, Lisandro Horacio
Beassoni, Paola Rita
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Pseudomonas aeruginosa phosphorylcholine phosphatase (PchP) catalyzes the hydrolysis of phosphorylcholine (Pcho), is activated by Mg2+ or Zn2+, and is inhibited by high concentrations of substrate.
This study has shown that PchP contains two sites for alkylammonium compounds (AACs): one in the catalytic site near the metal ion-phosphoester pocket, and the other in an inhibitory site responsible for the binding of the alkylammonium moiety.
The catalytic mechanism for the entry of Pcho in both sites and Zn2+ or Mg2+ follows a random sequential mechanism.
However, Zn2+ is more effective than Mg2+ at alleviating the inhibition produced by the entry of Pcho or different AACs in the inhibitory site.
We postulate that Zn2+ induces a conformational change in the active center that is communicated to the inhibitory site, producing a compact or closed structure.
In contrast, Mg2+ produces a relaxed or open conformation.
American Psychological Association (APA)
Otero, Lisandro Horacio& Beassoni, Paola Rita& Boetsch, Cristhian& Lisa, Angela Teresita& Domenech, Carlos Eduardo. 2011. Different Effects of Mg2+ and Zn2+ on the Two Sites for Alkylammonium Compounds in Pseudomonas aeruginosa Phosphorylcholine Phosphatase. Enzyme Research،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-508048
Modern Language Association (MLA)
Otero, Lisandro Horacio…[et al.]. Different Effects of Mg2+ and Zn2+ on the Two Sites for Alkylammonium Compounds in Pseudomonas aeruginosa Phosphorylcholine Phosphatase. Enzyme Research No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-508048
American Medical Association (AMA)
Otero, Lisandro Horacio& Beassoni, Paola Rita& Boetsch, Cristhian& Lisa, Angela Teresita& Domenech, Carlos Eduardo. Different Effects of Mg2+ and Zn2+ on the Two Sites for Alkylammonium Compounds in Pseudomonas aeruginosa Phosphorylcholine Phosphatase. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-508048
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508048