A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents
Joint Authors
Xiao, Xiaojin
Yang, Zhenzhen
Jiang, Mengli
Zhang, Lihong
Li, Xiaodong
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To bridge the convergence between traditional Chinese medicine (TCM) and modern medicine originated from the West, a new method of area under the absorbance-wavelength curve (AUAWC) by spectrophotometer scanning was investigated and compared with HPLC method to explore metabolomic pharmacokinetics in rats.
AUAWC and drug total concentration were obtained after Yangxue was injected to rats.
Meanwhile, individual concentrations of sodium ferulate, tetramethylpyrazine hydrochloride, tanshinol sodium, and sodium tanshinone IIA sulfonate in plasma were determined by HPLC.
Metabolomic profile of multicomponents plasma concentration time from AUAWC and that of individual components from HPLC were compared.
The data from AUAWC had one-compartment model with mean area under concentration versus time (AUC) of 9370.58 min·μg/mL and mean elimination half-life (t1/2) of 12.92 min.
The results by HPLC demonstrated that sodium ferulate and tetramethylpyrazine hydrochloride had one-compartment model with AUC of 6075.50 and 876.94 min·μg/mL, t1/2 of 10.85 and 20.57 min, respectively.
Tanshinol sodium and sodium tanshinone IIA sulfonate showed two-compartment model, and AUC was 29.58 and 201.46 with t1/2β of 1.76 and 16.90, respectively.
The profiles indicated that method of AUAWC can be used to study pharmacokinetics of TCM with multicomponents and to improve its development of active theory and application in clinic combined with in vivo metabolomic profile of HPLC.
American Psychological Association (APA)
Zhang, Lihong& Xiao, Xiaojin& Yang, Zhenzhen& Jiang, Mengli& Li, Xiaodong. 2013. A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents. Journal of Spectroscopy،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-508115
Modern Language Association (MLA)
Zhang, Lihong…[et al.]. A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents. Journal of Spectroscopy No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-508115
American Medical Association (AMA)
Zhang, Lihong& Xiao, Xiaojin& Yang, Zhenzhen& Jiang, Mengli& Li, Xiaodong. A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents. Journal of Spectroscopy. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-508115
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508115