Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea
Joint Authors
Ducki, Andrea
Heieren, Iris
Walbaum, Ulrike
Hoppert, Michael
Wrede, Christoph
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Methyl-Coenzyme M reductase (MCR) as key enzyme for methanogenesis as well as for anaerobic oxidation of methane represents an important metabolic marker for both processes in microbial biofilms.
Here, the potential of MCR-specific polyclonal antibodies as metabolic marker in various methanogenic Archaea is shown.
For standard growth conditions in laboratory culture, the cytoplasmic localization of the enzyme in Methanothermobacter marburgensis, Methanothermobacter wolfei, Methanococcus maripaludis, Methanosarcina mazei, and in anaerobically methane-oxidizing biofilms is demonstrated.
Under growth limiting conditions on nickel-depleted media, at low linear growth of cultures, a fraction of 50–70% of the enzyme was localized close to the cytoplasmic membrane, which implies “facultative” membrane association of the enzyme.
This feature may be also useful for assessment of growth-limiting conditions in microbial biofilms.
American Psychological Association (APA)
Wrede, Christoph& Walbaum, Ulrike& Ducki, Andrea& Heieren, Iris& Hoppert, Michael. 2013. Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea. Archaea،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-508193
Modern Language Association (MLA)
Wrede, Christoph…[et al.]. Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea. Archaea No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-508193
American Medical Association (AMA)
Wrede, Christoph& Walbaum, Ulrike& Ducki, Andrea& Heieren, Iris& Hoppert, Michael. Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea. Archaea. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-508193
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508193