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Effects of Resveratrol on Vitrified Porcine Oocytes
Joint Authors
Giaretta, Elisa
Tamanini, Carlo
Bucci, Diego
Galeati, Giovanna
Spinaci, Marcella
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Vitrified MII porcine oocytes are characterized by reduced developmental competence, associated with the activation of the apoptotic pathway.
Resveratrol (R), a polyphenolic compound present in several vegetal sources, has been reported to exert, among all its other biological effects, an antiapoptotic one.
The aim of this study was to determine the effects of R (2 µM) on the apoptotic status of porcine oocytes vitrified by Cryotop method, evaluating phosphatidylserine (PS) exteriorization and caspases activation.
R was added during IVM (A); 2 h postwarming incubation (B); vitrification/warming and 2 h postwarming incubation (C); all previous phases (D).
Data on PS exteriorization showed, in each treated group, a significantly higher (P<0.05) percentage of live nonapoptotic oocytes as compared with CTR; moreover, the percentage of live apoptotic oocytes was significantly (P<0.05) lower in all R-treated groups relative to CTR.
The results on caspase activation showed a tendency to an increase of viable oocytes with inactive caspases in B, C, and D, while a significant (P<0.05) increase in A compared to CTR was recorded.
These data demonstrate that R supplementation in various phases of IVM and vitrification/warming procedure can modulate the apoptotic process, improving the resistance of porcine oocytes to cryopreservation-induced damage.
American Psychological Association (APA)
Giaretta, Elisa& Spinaci, Marcella& Bucci, Diego& Tamanini, Carlo& Galeati, Giovanna. 2013. Effects of Resveratrol on Vitrified Porcine Oocytes. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-508194
Modern Language Association (MLA)
Giaretta, Elisa…[et al.]. Effects of Resveratrol on Vitrified Porcine Oocytes. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-508194
American Medical Association (AMA)
Giaretta, Elisa& Spinaci, Marcella& Bucci, Diego& Tamanini, Carlo& Galeati, Giovanna. Effects of Resveratrol on Vitrified Porcine Oocytes. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-508194
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508194