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Application of Ferriferous Oxide Modified by Chitosan in Gene Delivery
Joint Authors
Zhang, Zhongwei
Li, Mingyuan
Yang, Yuan
Yuan, Tun
Kuang, Yu
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
New approaches to improve the traditional gene carriers are still required.
Here we explore Fe3O4 modified with degradable polymers that enhances gene delivery and target delivery using permanent magnetic field.
Two magnetic Fe3O4 nanoparticles coated with chitosan (CTS) and polyethylene glycol (PEG) were synthesized by means of controlled chemical coprecipitation.
Plasmid pEGFP was encapsulated as a reported gene.
The ferriferous oxide complexes were approximately spherical; surface charge of CTS-Fe3O4 and PEG-Fe3O4 was about 20 mv and 0 mv, respectively.
The controlled release of DNA from the CTS-Fe3O4 nanoparticles was observed.
Concurrently, a desired Fe3O4 concentration of less than 2 mM was verified as safe by means of a cytotoxicity test in vitro.
Presence of the permanent magnetic field significantly increased the transfection efficiency.
Furthermore, the passive target property and safety of magnetic nanoparticles were also demonstrated in an in vivo test.
The novel gene delivery system was proved to be an effective tool required for future target expression and gene therapy in vivo.
American Psychological Association (APA)
Kuang, Yu& Yuan, Tun& Zhang, Zhongwei& Li, Mingyuan& Yang, Yuan. 2012. Application of Ferriferous Oxide Modified by Chitosan in Gene Delivery. Journal of Drug Delivery،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-508256
Modern Language Association (MLA)
Kuang, Yu…[et al.]. Application of Ferriferous Oxide Modified by Chitosan in Gene Delivery. Journal of Drug Delivery No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-508256
American Medical Association (AMA)
Kuang, Yu& Yuan, Tun& Zhang, Zhongwei& Li, Mingyuan& Yang, Yuan. Application of Ferriferous Oxide Modified by Chitosan in Gene Delivery. Journal of Drug Delivery. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-508256
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508256