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8-Prenylkaempferol Suppresses Influenza A Virus-Induced RANTES Production in A549 Cells via Blocking PI3K-Mediated Transcriptional Activation of NF-κB and IRF3
Joint Authors
Chen, Chen-Chih
Wei, Bai-Luh
Chiou, Wen-Fei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
8-Prenylkaempferol (8-PK) is a prenylflavonoid isolated from Sophora flavescens, a Chinese herb with antiviral and anti-inflammatory properties.
In this study, we investigated its effect on regulated activation, normal T cell expressed and secreted (RANTES) secretion by influenza A virus (H1N1)-infected A549 alveolar epithelial cells.
Cell inoculation with H1N1 evoked a significant induction in RANTES accumulation accompanied with time-related increase in nuclear translocation of nuclear factor-κB (NF-κB) and interferon regulatory factor 3 (IRF-3), but showed no effect on c-Jun phosphorylation.
8-PK could significantly inhibit not only RANTES production but also NF-κB and IRF-3 nuclear translocation.
We had proved that both NF-κB and IRF-3 participated in H1N1-induced RANTES production since NF-κB inhibitor pyrrolidinedithio carbamate (PDTC) and IRF-3 siRNA attenuated significantly RANTES accumulation.
H1N1 inoculation also increased PI3K activity as well as Akt phosphorylation and such responsiveness were attenuated by 8-PK.
In the presence of wortmannin, nuclear translocation of NF-κB and IRF3 as well as RANTES production by H1N1 infection were all reversed, demonstrating that PI3K-Akt pathway is essential for NF-κB- and IRF-3-mediated RANTES production in A549 cells.
Furthermore, 8-PK but not wortmannin, prevented effectively H1N1-evoked IκB degradation.
In conclusion, 8-PK might be an anti-inflammatory agent for suppressing influenza A virus-induced RANTES production acts by blocking PI3K-mediated transcriptional activation of NF-κB and IRF-3 and in part by interfering with IκB degradation which subsequently decreases NF-κB translocation.
American Psychological Association (APA)
Chiou, Wen-Fei& Chen, Chen-Chih& Wei, Bai-Luh. 2011. 8-Prenylkaempferol Suppresses Influenza A Virus-Induced RANTES Production in A549 Cells via Blocking PI3K-Mediated Transcriptional Activation of NF-κB and IRF3. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-508261
Modern Language Association (MLA)
Chiou, Wen-Fei…[et al.]. 8-Prenylkaempferol Suppresses Influenza A Virus-Induced RANTES Production in A549 Cells via Blocking PI3K-Mediated Transcriptional Activation of NF-κB and IRF3. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-508261
American Medical Association (AMA)
Chiou, Wen-Fei& Chen, Chen-Chih& Wei, Bai-Luh. 8-Prenylkaempferol Suppresses Influenza A Virus-Induced RANTES Production in A549 Cells via Blocking PI3K-Mediated Transcriptional Activation of NF-κB and IRF3. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-508261
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508261