Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis
Joint Authors
Calzado, Enrique Rengifo
Cedeño, Mercedes
Carr, Adriana
Lamadrid, Janet
Gutiérrez, Zailí
Rengifo, Charles E.
Blanco, Rancés
Frómeta, Milagros
Osorio, Marta
Escobar, Xiomara
Quintana, Yisel
Blanco, Damián
Source
Pathology Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The reactivity of the 14F7 Mab, a highly specific IgG1 against N-glycolyl GM3 ganglioside (NeuGcGM3) in normal tissues, lymphomas, lymph node metastasis, and other metastatic sites was assessed by immunohistochemistry.
In addition, the effect of chemical fixation on the 14F7 Mab staining using monolayers of P3X63Ag.653 cells was also evaluated.
Moreover, the ability of 14F7 to bind NeuGcGM3 ganglioside inducing complement-independent cytotoxicity by a flow cytometry-based assay was measured.
The 14F7 Mab was reactive in unfixed, 4% paraformaldehyde, 4% formaldehyde, and acetone fixed cells.
Postfixation with acetone did not alter the localization of NeuGcGM3, while the staining with 14F7 Mab was significantly eliminated in both cells fixed and postfixed with methanol but only partially reduced with ethanol.
The staining with 14F7 Mab was evidenced in the 89.2%, 89.4%, and 88.9% of lymphomas, lymph node metastasis, and other metastatic sites, respectively, but not in normal tissues.
The treatment with 14F7 Mab affected both morphology and membrane integrity of P3X63Ag.653 cells.
This cytotoxic activity was dose-dependent and ranged from 24.0 to 84.7% (10–1000 μg/mL) as compared to the negative control.
Our data could support the possible use of NeuGcGM3 as target for both active and passive immunotherapy against malignancies expressing this molecule.
American Psychological Association (APA)
Blanco, Rancés& Blanco, Damián& Quintana, Yisel& Escobar, Xiomara& Rengifo, Charles E.& Osorio, Marta…[et al.]. 2013. Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis. Pathology Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-508283
Modern Language Association (MLA)
Blanco, Rancés…[et al.]. Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis. Pathology Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-508283
American Medical Association (AMA)
Blanco, Rancés& Blanco, Damián& Quintana, Yisel& Escobar, Xiomara& Rengifo, Charles E.& Osorio, Marta…[et al.]. Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis. Pathology Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-508283
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508283