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Quercetin and Sesamin Protect Dopaminergic Cells from MPP+-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System
Joint Authors
Plouffe, Marilyn
Bournival, Julie
Martinoli, Maria-Grazia
Renaud, Justine
Provencher, Cindy
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
A growing body of evidence indicates that the majority of Parkinson’s disease (PD) cases are associated with microglia activation with resultant elevation of various inflammatory mediators and neuroinflammation.
In this study, we investigated the effects of 2 natural molecules, quercetin and sesamin, on neuroinflammation induced by the Parkinsonian toxin 1-methyl-4-phenylpyridinium (MPP+) in a glial-neuronal system.
We first established that quercetin and sesamin defend microglial cells against MPP+-induced increases in the mRNA or protein levels of 3 pro-inflammatory cytokines (interleukin-6, IL-1β and tumor necrosis factor-alpha), as revealed by real time-quantitative polymerase chain reaction and enzyme-linked immunoabsorbent assay, respectively.
Quercetin and sesamin also decrease MPP+-induced oxidative stress in microglial cells by reducing inducible nitric oxide synthase protein expression as well as mitochondrial superoxide radicals.
We then measured neuronal cell death and apoptosis after MPP+ activation of microglia, in a microglial (N9)-neuronal (PC12) coculture system.
Our results revealed that quercetin and sesamin rescued neuronal PC12 cells from apoptotic death induced by MPP+ activation of microglial cells.
Altogether, our data demonstrate that the phytoestrogen quercetin and the lignan sesamin diminish MPP+-evoked microglial activation and suggest that both these molecules may be regarded as potent, natural, anti-inflammatory compounds.
American Psychological Association (APA)
Bournival, Julie& Plouffe, Marilyn& Renaud, Justine& Provencher, Cindy& Martinoli, Maria-Grazia. 2012. Quercetin and Sesamin Protect Dopaminergic Cells from MPP+-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-508365
Modern Language Association (MLA)
Bournival, Julie…[et al.]. Quercetin and Sesamin Protect Dopaminergic Cells from MPP+-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-508365
American Medical Association (AMA)
Bournival, Julie& Plouffe, Marilyn& Renaud, Justine& Provencher, Cindy& Martinoli, Maria-Grazia. Quercetin and Sesamin Protect Dopaminergic Cells from MPP+-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-508365
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508365