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Hepatic Differentiation of Murine Disease-Specific Induced Pluripotent Stem Cells Allows Disease Modelling In Vitro
Joint Authors
Cantz, Tobias
Sgodda, Malte
Loya, Komal
Eggenschwiler, Reto
André, Francoise
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Direct reprogramming of somatic cells into pluripotent cells by retrovirus-mediated expression of OCT4, SOX2, KLF4, and C-MYC is a promising approach to derive disease-specific induced pluripotent stem cells (iPSCs).
In this study, we focused on three murine models for metabolic liver disorders: the copper storage disorder Wilson's disease (toxic-milk mice), tyrosinemia type 1 (fumarylacetoacetate-hydrolase deficiency, FAH−/− mice), and alpha1-antitrypsin deficiency (PiZ mice).
Colonies of iPSCs emerged 2-3 weeks after transduction of fibroblasts, prepared from each mouse strain, and were maintained as individual iPSC lines.
RT-PCR and immunofluorescence analyses demonstrated the expression of endogenous pluripotency markers.
Hepatic precursor cells could be derived from these disease-specific iPSCs applying an in vitro differentiation protocol and could be visualized after transduction of a lentiviral albumin-GFP reporter construct.
Functional characterization of these cells allowed the recapitulation of the disease phenotype for further studies of underlying molecular mechanisms of the respective disease.
American Psychological Association (APA)
Eggenschwiler, Reto& Loya, Komal& Sgodda, Malte& André, Francoise& Cantz, Tobias. 2011. Hepatic Differentiation of Murine Disease-Specific Induced Pluripotent Stem Cells Allows Disease Modelling In Vitro. Stem Cells International،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-508523
Modern Language Association (MLA)
Eggenschwiler, Reto…[et al.]. Hepatic Differentiation of Murine Disease-Specific Induced Pluripotent Stem Cells Allows Disease Modelling In Vitro. Stem Cells International No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-508523
American Medical Association (AMA)
Eggenschwiler, Reto& Loya, Komal& Sgodda, Malte& André, Francoise& Cantz, Tobias. Hepatic Differentiation of Murine Disease-Specific Induced Pluripotent Stem Cells Allows Disease Modelling In Vitro. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-508523
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508523