Spectroscopic Characterization of Intermolecular Interaction of Amyloid β Promoted on GM1 Micelles

Joint Authors

Gekko, Kunihiko
Yanagisawa, Katsuhiko
Matsuo, Koichi
Yagi-Utsumi, Maho
Kato, Koichi

Source

International Journal of Alzheimer's Disease

Issue

Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2010-12-28

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Diseases
Medicine

Abstract EN

Clusters of GM1 gangliosides act as platforms for conformational transition of monomeric, unstructured amyloid β (Aβ) to its toxic β-structured aggregates.

We have previously shown that Aβ(1–40) accommodated on the hydrophobic/hydrophilic interface of lyso-GM1 or GM1 micelles assumes α-helical structures under ganglioside-excess conditions.

For better understanding of the mechanisms underlying the α-to-β conformational transition of Aβ on GM1 clusters, we performed spectroscopic characterization of Aβ(1–40) titrated with GM1.

It was revealed that the thioflavin T- (ThT-) reactive β-structure is more populated in Aβ(1–40) under conditions where the Aβ(1–40) density on GM1 micelles is high.

Under this circumstance, the C-terminal hydrophobic anchor Val39-Val40 shows two distinct conformational states that are reactive with ThT, while such Aβ species were not generated by smaller lyso-GM1 micelles.

These findings suggest that GM1 clusters promote specific Aβ-Aβ interactions through their C-termini coupled with formation of the ThT-reactive β-structure depending on sizes and curvatures of the clusters.

American Psychological Association (APA)

Yagi-Utsumi, Maho& Matsuo, Koichi& Yanagisawa, Katsuhiko& Gekko, Kunihiko& Kato, Koichi. 2010. Spectroscopic Characterization of Intermolecular Interaction of Amyloid β Promoted on GM1 Micelles. International Journal of Alzheimer's Disease،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-508556

Modern Language Association (MLA)

Yagi-Utsumi, Maho…[et al.]. Spectroscopic Characterization of Intermolecular Interaction of Amyloid β Promoted on GM1 Micelles. International Journal of Alzheimer's Disease No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-508556

American Medical Association (AMA)

Yagi-Utsumi, Maho& Matsuo, Koichi& Yanagisawa, Katsuhiko& Gekko, Kunihiko& Kato, Koichi. Spectroscopic Characterization of Intermolecular Interaction of Amyloid β Promoted on GM1 Micelles. International Journal of Alzheimer's Disease. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-508556

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-508556