Isolation and Genetic Analysis of Bovine Viral Diarrhea Virus from Infected Cattle in Indiana

Abstract EN

Species and biotype distribution was determined in 44 bovine viral diarrhea virus- (BVDV-) positive samples submitted to the Animal Disease Diagnostic Laboratory (ADDL) in Indiana during 2006–2008.

BVDV RNA was detected in the 5′-untranslated region and Npro region using reverse transcriptase PCR followed by sequencing analysis of the PCR product.

Additionally, cases were classified into one of six categories according to history and/or lesions: acute symptomatic, hemorrhagic, respiratory distress, reproductive, persistent infection (PI), and mucosal disease (MD).

Of 44 BVDV-positive samples, 33 were noncytopathic (ncp), 10 were cytopathic (cp), and one presented both ncp and cp biotypes.

Sequencing analysis demonstrated that all samples belonged to BVDV-1a, BVDV-1b, or BVDV-2.

The most common isolate was ncp BVDV-1b, (44%) followed by ncp BVDV-2a (24%).

Among the six categories, respiratory clinical signs were the most common (36%) followed by PI (25%) and MD (16%).