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Isolation and Genetic Analysis of Bovine Viral Diarrhea Virus from Infected Cattle in Indiana
Joint Authors
Murphy, Duane A.
Negron, Maria
Raizman, Eran A.
Thacker, H. Leon
Pogranichniy, Roman M.
Schnur, Megan E.
Source
Veterinary Medicine International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Species and biotype distribution was determined in 44 bovine viral diarrhea virus- (BVDV-) positive samples submitted to the Animal Disease Diagnostic Laboratory (ADDL) in Indiana during 2006–2008.
BVDV RNA was detected in the 5′-untranslated region and Npro region using reverse transcriptase PCR followed by sequencing analysis of the PCR product.
Additionally, cases were classified into one of six categories according to history and/or lesions: acute symptomatic, hemorrhagic, respiratory distress, reproductive, persistent infection (PI), and mucosal disease (MD).
Of 44 BVDV-positive samples, 33 were noncytopathic (ncp), 10 were cytopathic (cp), and one presented both ncp and cp biotypes.
Sequencing analysis demonstrated that all samples belonged to BVDV-1a, BVDV-1b, or BVDV-2.
The most common isolate was ncp BVDV-1b, (44%) followed by ncp BVDV-2a (24%).
Among the six categories, respiratory clinical signs were the most common (36%) followed by PI (25%) and MD (16%).
American Psychological Association (APA)
Pogranichniy, Roman M.& Schnur, Megan E.& Raizman, Eran A.& Murphy, Duane A.& Negron, Maria& Thacker, H. Leon. 2011. Isolation and Genetic Analysis of Bovine Viral Diarrhea Virus from Infected Cattle in Indiana. Veterinary Medicine International،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-508629
Modern Language Association (MLA)
Pogranichniy, Roman M.…[et al.]. Isolation and Genetic Analysis of Bovine Viral Diarrhea Virus from Infected Cattle in Indiana. Veterinary Medicine International No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-508629
American Medical Association (AMA)
Pogranichniy, Roman M.& Schnur, Megan E.& Raizman, Eran A.& Murphy, Duane A.& Negron, Maria& Thacker, H. Leon. Isolation and Genetic Analysis of Bovine Viral Diarrhea Virus from Infected Cattle in Indiana. Veterinary Medicine International. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-508629
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508629