Construction and Characterization of an Infectious Murine Gammaherpesivrus-68 Bacterial Artificial Chromosome
Joint Authors
Smith, Greg
Liao, Hsiang-I
Leang, Ronika Sitapara
Sun, Ren
Tong, Leming
Wu, Ting-Ting
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Here we describe the cloning of a sequenced WUMS isolate of murine gammaherpesvirus-68 (MHV-68, γHV-68, also known as MuHV-4) as a bacterial artificial chromosome (BAC).
We engineered the insertion of the BAC sequence flanked by loxP sites into the left end of the viral genome before the M1 open reading frame.
The infectious viruses were reconstituted following transfection of the MHV-68 BAC DNA into cells.
The MHV-68 BAC-derived virus replicated indistinguishably from the wild-type virus in cultured cells.
Excision of the BAC insert was efficiently achieved by coexpressing the Cre recombinase.
Although the BAC insertion did not significantly affect acute productive infection in the lung, it severely compromised the ability of MHV-68 to establish splenic latency.
Removal of the BAC sequence restored the wild-type level of latency.
Site-specific mutagenesis was carried out by RecA-mediated recombination to demonstrate that this infectious BAC clone can be used for genetic studies of MHV-68.
American Psychological Association (APA)
Wu, Ting-Ting& Liao, Hsiang-I& Tong, Leming& Leang, Ronika Sitapara& Smith, Greg& Sun, Ren. 2010. Construction and Characterization of an Infectious Murine Gammaherpesivrus-68 Bacterial Artificial Chromosome. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-508664
Modern Language Association (MLA)
Wu, Ting-Ting…[et al.]. Construction and Characterization of an Infectious Murine Gammaherpesivrus-68 Bacterial Artificial Chromosome. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-508664
American Medical Association (AMA)
Wu, Ting-Ting& Liao, Hsiang-I& Tong, Leming& Leang, Ronika Sitapara& Smith, Greg& Sun, Ren. Construction and Characterization of an Infectious Murine Gammaherpesivrus-68 Bacterial Artificial Chromosome. Journal of Biomedicine and Biotechnology. 2010. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-508664
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508664