Rosiglitazone and Pioglitazone Alter Aromatase Kinetic Properties in Human Granulosa Cells
Joint Authors
Araki, Takako
Rosenwaks, Zev
Varadinova, Miroslava
Poretsky, Leonid
Goldman, Michael
Seto-Young, Donna
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
We have previously reported that, in human granulosa cells, thiazolidinediones rosiglitazone and pioglitazone inhibit estrogen synthesis by interfering with androgen binding to aromatase, without an effect on aromatase mRNA or protein expression.
In the current paper, we explore the effects of rosiglitazone and pioglitazone on the aromatase enzyme kinetic properties in human granulosa cells.
The cells were incubated with various concentrations of testosterone or androstenedione, with or without rosiglitazone or pioglitazone.
Estradiol and estrone concentrations in the conditioned tissue culture medium were measured by radioimmunoassay or immunosorbent assay.
When testosterone was used as substrate, rosiglitazone or pioglitazone inhibited the Vmax by 35% (P<0.001) and 24% (P<0.001), respectively.
When androstenedione was used as substrate, both rosiglitazone or pioglitazone inhibited Vmax by 13% (P<0.007).
We conclude that rosiglitazone or pioglitazone has no effect on Km but inhibits Vmax of aromatase in human granulosa cells, therefore, acting as noncompetitive inhibitors.
American Psychological Association (APA)
Araki, Takako& Varadinova, Miroslava& Goldman, Michael& Rosenwaks, Zev& Poretsky, Leonid& Seto-Young, Donna. 2011. Rosiglitazone and Pioglitazone Alter Aromatase Kinetic Properties in Human Granulosa Cells. PPAR Research،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-508692
Modern Language Association (MLA)
Araki, Takako…[et al.]. Rosiglitazone and Pioglitazone Alter Aromatase Kinetic Properties in Human Granulosa Cells. PPAR Research No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-508692
American Medical Association (AMA)
Araki, Takako& Varadinova, Miroslava& Goldman, Michael& Rosenwaks, Zev& Poretsky, Leonid& Seto-Young, Donna. Rosiglitazone and Pioglitazone Alter Aromatase Kinetic Properties in Human Granulosa Cells. PPAR Research. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-508692
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508692