Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease
Joint Authors
Hungerford, Valerie
Case, Stephanie
Zou, Chao
Luyten, Marcel
Michels, Aaron W.
Yu, Liping
Seidl, Katherine J.
Gerhartz, Bernd
Liu, Edwin
Fankhauser, Gabriela
Ostermann, Nils
McDaniel, Kristen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Class II major histocompatibility molecules confer disease risk in Celiac disease (CD) by presenting gliadin peptides to CD4 T cells in the small intestine.
Deamidation of gliadin peptides by tissue transglutaminase creates immunogenic peptides presented by HLA-DQ2 and DQ8 molecules to activate proinflammatory CD4 T cells.
Detecting gliadin specific T cell responses from the peripheral blood has been challenging due to low circulating frequencies and heterogeneity in response to gliadin epitopes.
We investigated the peripheral T cell responses to alpha and gamma gliadin epitopes in young children with newly diagnosed and untreated CD.
Using peptide/MHC recombinant protein constructs, we are able to robustly stimulate CD4 T cell clones previously derived from intestinal biopsies of CD patients.
These recombinant proteins and a panel of α- and γ-gliadin peptides were used to assess T cell responses from the peripheral blood.
Proliferation assays using peripheral blood mononuclear cells revealed more CD4 T cell responses to α-gliadin than γ-gliadin peptides with a single deamidated α-gliadin peptide able to identify 60% of CD children.
We conclude that it is possible to detect T cell responses without a gluten challenge or in vitro stimulus other than antigen, when measuring proliferative responses.
American Psychological Association (APA)
Liu, Edwin& McDaniel, Kristen& Case, Stephanie& Yu, Liping& Gerhartz, Bernd& Ostermann, Nils…[et al.]. 2014. Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease. Autoimmune Diseases،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-508754
Modern Language Association (MLA)
Liu, Edwin…[et al.]. Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease. Autoimmune Diseases No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-508754
American Medical Association (AMA)
Liu, Edwin& McDaniel, Kristen& Case, Stephanie& Yu, Liping& Gerhartz, Bernd& Ostermann, Nils…[et al.]. Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease. Autoimmune Diseases. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-508754
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508754