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Spectroscopic Investigations of Pentobarbital Interaction with Transthyretin
Joint Authors
Darwish, Saqer M.
Faroun, Mariam
Ghithan, Jafar
Abu-hadid, Mahmoud M.
Abuteir, Musa M.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Transthyretin (TTR) aggregation has been characterized to be responsible for several amyloid diseases.
Fourier transform infrared (FTIR) spectroscopy, fluorescence, and atomic force microscopy (AFM) are used to investigate secondary structure changes in transthyretin, induced upon thermal denaturation and interaction with pentobarbital.
Spectral analysis revealed a strong static quenching of the intrinsic fluorescence of TTR by pentobarbital with a binding constant (K) estimated at 2.092×103 M-1.
Fourier self-deconvolution (FSD) technique is used to evaluates intensity changes in the spectra of the component bands in the amide I and amide II regions due to the changes in pentobarbital concentration in the protein complex.
The increases of the relative intensities of the peaks at 1614 cm−1 and 1507 cm−1 are due to the increase of pentobarbital concentrations which is linked to the formation of oligomers in the protein.
American Psychological Association (APA)
Darwish, Saqer M.& Ghithan, Jafar& Abuteir, Musa M.& Faroun, Mariam& Abu-hadid, Mahmoud M.. 2012. Spectroscopic Investigations of Pentobarbital Interaction with Transthyretin. Journal of Spectroscopy،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-508837
Modern Language Association (MLA)
Darwish, Saqer M.…[et al.]. Spectroscopic Investigations of Pentobarbital Interaction with Transthyretin. Journal of Spectroscopy No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-508837
American Medical Association (AMA)
Darwish, Saqer M.& Ghithan, Jafar& Abuteir, Musa M.& Faroun, Mariam& Abu-hadid, Mahmoud M.. Spectroscopic Investigations of Pentobarbital Interaction with Transthyretin. Journal of Spectroscopy. 2012. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-508837
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508837