Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro
Joint Authors
Dominguez, Michel
Bendayan, Moise
Levy, Émile
Cammisotto, Philippe G.
Garofalo, Carole
Sané, Alain
Source
International Journal of Cell Biology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-04-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Gastric Leptin is absorbed by duodenal enterocytes and released on the basolateral side towards the bloodstream.
We investigated in vitro some of the mechanisms of this transport.
Caco-2/15 cells internalize leptin from the apical medium and release it through transcytosis in the basal medium in a time- temperature-dependent and saturable fashion.
Leptin receptors are revealed on the apical brush-border membrane of the Caco-2 cells.
RNA-mediated silencing of the receptor led to decreases in the uptake and basolateral release.
Leptin in the basal medium was found bound to the soluble form of its receptor.
An inhibitor of clathrin-dependent endocytosis (chlorpromazine) decreased leptin uptake.
Confocal immunocytochemistry and the use of brefeldin A and okadaic acid revealed the passage of leptin through the Golgi apparatus.
We propose that leptin transcytosis by intestinal cells depends on its receptor, on clathrin-coated vesicles and transits through the Golgi apparatus.
American Psychological Association (APA)
Cammisotto, Philippe G.& Bendayan, Moise& Sané, Alain& Dominguez, Michel& Garofalo, Carole& Levy, Émile. 2010. Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro. International Journal of Cell Biology،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-508865
Modern Language Association (MLA)
Cammisotto, Philippe G.…[et al.]. Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro. International Journal of Cell Biology No. 2010 (2010), pp.1-13.
https://search.emarefa.net/detail/BIM-508865
American Medical Association (AMA)
Cammisotto, Philippe G.& Bendayan, Moise& Sané, Alain& Dominguez, Michel& Garofalo, Carole& Levy, Émile. Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro. International Journal of Cell Biology. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-508865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508865