Anticancer Activity of Indian Stingless Bee Propolis : An In Vitro Study
Joint Authors
Rajwade, Jyutika M.
Haghniaz, Reihaneh
Paknikar, Kishore M.
Choudhari, Milind K.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Indian stingless bee propolis has a complex chemical nature and is reported to possess various medicinal properties.
In the present study, anticancer activity of the ethanolic extract of propolis (EEP) was explored by testing the cytotoxic and apoptotic effect in four different cancer cell lines, namely, MCF-7 (human breast cancer), HT-29 (human colon adenocarcinoma), Caco-2 (human epithelial colorectal adenocarcinoma), and B16F1 (murine melanoma), at different concentrations.
Cytotoxicity was evaluated by MTT assay and Trypan blue dye exclusion assay.
EEP at a concentration of 250 μg/mL exhibited ≥50% mortality in all cell lines tested (i.e., IC50 value).
EEP revealed a concentration and time dependent cytotoxic effect.
Apoptosis was estimated by differential staining (ethidium bromide/acridine orange) and TUNEL (deoxynucleotidyl transferase-dUTP nick end labeling) assay.
Light microscopy and atomic force microscopy demonstrated morphological features of apoptosis in all the cell lines after treatment with 250 μg/mL EEP for 24 h.
Thus, early onset of apoptosis is the reason for anticancer activity of Indian stingless bee propolis.
Further, the antioxidant potential of Indian stingless bee propolis was demonstrated to substantiate its anticancer activity.
American Psychological Association (APA)
Choudhari, Milind K.& Haghniaz, Reihaneh& Rajwade, Jyutika M.& Paknikar, Kishore M.. 2013. Anticancer Activity of Indian Stingless Bee Propolis : An In Vitro Study. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-508873
Modern Language Association (MLA)
Choudhari, Milind K.…[et al.]. Anticancer Activity of Indian Stingless Bee Propolis : An In Vitro Study. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-508873
American Medical Association (AMA)
Choudhari, Milind K.& Haghniaz, Reihaneh& Rajwade, Jyutika M.& Paknikar, Kishore M.. Anticancer Activity of Indian Stingless Bee Propolis : An In Vitro Study. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-508873
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508873