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Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs
Joint Authors
Lin, Yih Jyh
Tai, Hao-Chih
Hara, Hidetaka
Quader, Mubina A.
Zhu, Xiaocheng
Epperley, Michael
Cooper, David K. C.
Ezzelarab, Mohamed
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Allograft/xenograft rejection is associated with “passenger leukocyte” migration from the organ into recipient lymph nodes.
In Study 1, we attempted to deplete leukocytes from potential kidney “donor” pigs, using two regimens of total body irradiation.
A dose of 700 cGy was administered, followed by either 800 cGy (“low-dose”) or 1,300 cGy (“high dose”) with the kidneys shielded.
Neither regimen was entirely successful in depleting all leukocytes, although remaining T and 8 cell numbers were negligible.
Study 2 was aimed at providing an indication of whether near-complete depletion of leukocytes had any major impact on kidney allograft survival.
In non-immunosuppressed recipient pigs, survival of a kidney from a donor that received high-dose irradiation was compared with that of a kidney taken from a non-irradiated donor.
Kidney graft survival was 9 and 7 days, respectively, suggesting that depletion had little impact on graft survival.
The lack of effect may have been related to (i) inadequate depletion of passenger leukocytes, thus not preventing a direct T cell response, (ii) the presence of dead or dying leukocytes (antigens), thus not preventing an indirect T cell response, or (iii) constitutive expression of MHC class II and B7 molecules on the porcine vascular endothelium, activating recipient T cells.
American Psychological Association (APA)
Tai, Hao-Chih& Zhu, Xiaocheng& Lin, Yih Jyh& Hara, Hidetaka& Ezzelarab, Mohamed& Epperley, Michael…[et al.]. 2011. Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs. Journal of Transplantation،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-508926
Modern Language Association (MLA)
Tai, Hao-Chih…[et al.]. Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs. Journal of Transplantation No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-508926
American Medical Association (AMA)
Tai, Hao-Chih& Zhu, Xiaocheng& Lin, Yih Jyh& Hara, Hidetaka& Ezzelarab, Mohamed& Epperley, Michael…[et al.]. Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs. Journal of Transplantation. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-508926
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508926