Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs

Joint Authors

Lin, Yih Jyh
Tai, Hao-Chih
Hara, Hidetaka
Quader, Mubina A.
Zhu, Xiaocheng
Epperley, Michael
Cooper, David K. C.
Ezzelarab, Mohamed

Source

Journal of Transplantation

Issue

Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-12-18

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Medicine

Abstract EN

Allograft/xenograft rejection is associated with “passenger leukocyte” migration from the organ into recipient lymph nodes.

In Study 1, we attempted to deplete leukocytes from potential kidney “donor” pigs, using two regimens of total body irradiation.

A dose of 700 cGy was administered, followed by either 800 cGy (“low-dose”) or 1,300 cGy (“high dose”) with the kidneys shielded.

Neither regimen was entirely successful in depleting all leukocytes, although remaining T and 8 cell numbers were negligible.

Study 2 was aimed at providing an indication of whether near-complete depletion of leukocytes had any major impact on kidney allograft survival.

In non-immunosuppressed recipient pigs, survival of a kidney from a donor that received high-dose irradiation was compared with that of a kidney taken from a non-irradiated donor.

Kidney graft survival was 9 and 7 days, respectively, suggesting that depletion had little impact on graft survival.

The lack of effect may have been related to (i) inadequate depletion of passenger leukocytes, thus not preventing a direct T cell response, (ii) the presence of dead or dying leukocytes (antigens), thus not preventing an indirect T cell response, or (iii) constitutive expression of MHC class II and B7 molecules on the porcine vascular endothelium, activating recipient T cells.

American Psychological Association (APA)

Tai, Hao-Chih& Zhu, Xiaocheng& Lin, Yih Jyh& Hara, Hidetaka& Ezzelarab, Mohamed& Epperley, Michael…[et al.]. 2011. Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs. Journal of Transplantation،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-508926

Modern Language Association (MLA)

Tai, Hao-Chih…[et al.]. Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs. Journal of Transplantation No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-508926

American Medical Association (AMA)

Tai, Hao-Chih& Zhu, Xiaocheng& Lin, Yih Jyh& Hara, Hidetaka& Ezzelarab, Mohamed& Epperley, Michael…[et al.]. Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs. Journal of Transplantation. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-508926

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-508926