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An Elegant Biosensor Molecular Beacon Probe : Challenges and Recent Solutions
Author
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-13
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Diseases
Abstract EN
Molecular beacon (MB) probes are fluorophore- and quencher-labeled short synthetic DNAs folded in a stem-loop shape.
Since the first report by Tyagi and Kramer, it has become a widely accepted tool for nucleic acid analysis and triggered a cascade of related developments in the field of molecular sensing.
The unprecedented success of MB probes stems from their ability to detect specific DNA or RNA sequences immediately after hybridization with no need to wash out the unbound probe (instantaneous format).
Importantly, the hairpin structure of the probe is responsible for both the low fluorescent background and improved selectivity.
Furthermore, the signal is generated in a reversible manner; thus, if the analyte is removed, the signal is reduced to the background.
This paper highlights the advantages of MB probes and discusses the approaches that address the challenges in MB probe design.
Variations of MB-based assays tackle the problem of stem invasion, improve SNP genotyping and signal-to-noise ratio, as well as address the challenges of detecting folded RNA and DNA.
American Psychological Association (APA)
Kolpashchikov, Dmitry M.. 2012. An Elegant Biosensor Molecular Beacon Probe : Challenges and Recent Solutions. Scientifica،Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-508928
Modern Language Association (MLA)
Kolpashchikov, Dmitry M.. An Elegant Biosensor Molecular Beacon Probe : Challenges and Recent Solutions. Scientifica No. 2012 (2012), pp.1-17.
https://search.emarefa.net/detail/BIM-508928
American Medical Association (AMA)
Kolpashchikov, Dmitry M.. An Elegant Biosensor Molecular Beacon Probe : Challenges and Recent Solutions. Scientifica. 2012. Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-508928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508928