Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)
Joint Authors
Lin, Peng
Wong, Jack Ho
Ng, Tzi Bun
Sharma, Arishya
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-03-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A lectin has been isolated from seeds of the Phaseolus vulgaris cv.
“Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200.
The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins.
The hemagglutinating activity of the lectin was stable within the pH range of 1–14 and the temperature range of 0–80∘C.
The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC50 of 1.3 μM, and inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 7.6 μM.
The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation.
The lectin had no antifungal activity.
It did not stimulate nitric oxide production by murine peritoneal macrophages.
Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin.
American Psychological Association (APA)
Sharma, Arishya& Ng, Tzi Bun& Wong, Jack Ho& Lin, Peng. 2009. Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans). Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-508994
Modern Language Association (MLA)
Sharma, Arishya…[et al.]. Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans). Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-508994
American Medical Association (AMA)
Sharma, Arishya& Ng, Tzi Bun& Wong, Jack Ho& Lin, Peng. Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans). Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-508994
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508994