Leaves and Seeds as Materials for Flow Cytometric Estimation of the Genome Size of 11 Rosaceae Woody Species Containing DNA-Staining Inhibitors
Joint Authors
Jedrzejczyk, Iwona
Sliwinska, Elwira
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The presence of some secondary metabolites in the cell cytosol can cause a stoichiometric error in the flow cytometric estimation of nuclear DNA content.
There is no fully reliable method to completely eliminate the effect of these compounds on nuclei fluorescence, and therefore using plant organs/parts free of staining inhibitors is recommended.
Eleven species of Rosaceae with high concentrations of propidium-iodide-staining inhibitors were studied to check the possibility of using seeds instead of leaves for genome size estimation.
Despite optimizing the concentration and composition of antioxidants in nuclei-isolation buffer for each species, the effect of cytosolic compounds present in the leaves could not be avoided entirely.
None of the seeds of the studied species contained inhibitors, and they produced histograms of good quality.
The genome size of the studied species ranged from 1.15 to 3.17 pg/2C; for 10 species the DNA content was estimated for the first time.
American Psychological Association (APA)
Jedrzejczyk, Iwona& Sliwinska, Elwira. 2010. Leaves and Seeds as Materials for Flow Cytometric Estimation of the Genome Size of 11 Rosaceae Woody Species Containing DNA-Staining Inhibitors. Journal of Botany،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-509109
Modern Language Association (MLA)
Jedrzejczyk, Iwona& Sliwinska, Elwira. Leaves and Seeds as Materials for Flow Cytometric Estimation of the Genome Size of 11 Rosaceae Woody Species Containing DNA-Staining Inhibitors. Journal of Botany No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-509109
American Medical Association (AMA)
Jedrzejczyk, Iwona& Sliwinska, Elwira. Leaves and Seeds as Materials for Flow Cytometric Estimation of the Genome Size of 11 Rosaceae Woody Species Containing DNA-Staining Inhibitors. Journal of Botany. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-509109
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509109