Hypomethylation of IL10 and IL13 Promoters in CD4+ T Cells of Patients with Systemic Lupus Erythematosus
Joint Authors
Lu, Qianjin
Gao, Fei
Tang, Jinling
Liang, Yunsheng
Zhao, Ming
Yin, Heng
Wu, Xiaoyan
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Interleukin- (IL-)10 and IL-13 play important roles in Th2 cell differentiation and production of autoantibodies in patients with (SLE).
However, the mechanisms leading to IL10 and IL13 overexpression in SLE patients are not well understood.
In this study, we confirm that the levels of both IL10 and IL13 mRNA in CD4+ T cells and of serum IL10 and IL13 proteins are increased in SLE patients.
We show that the DNA methylation levels within IL10 and IL13 gene regulatory domains are reduced in SLE CD4+ T cells relative to healthy controls and negatively correlate with IL10 and IL13 mRNA expression.
Moreover, treating healthy CD4+ T cells with the demethylating agent 5-azacytidine (5-azaC) increased IL10 and IL13 mRNA transcription.
Together, our results show that promoter methylation is a determinant of IL10 and IL13 expression in CD4+ T cells, and we propose that DNA hypomethylation leads to IL10 and IL13 overexpression in SLE patients.
American Psychological Association (APA)
Zhao, Ming& Tang, Jinling& Gao, Fei& Wu, Xiaoyan& Liang, Yunsheng& Yin, Heng…[et al.]. 2010. Hypomethylation of IL10 and IL13 Promoters in CD4+ T Cells of Patients with Systemic Lupus Erythematosus. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-509125
Modern Language Association (MLA)
Zhao, Ming…[et al.]. Hypomethylation of IL10 and IL13 Promoters in CD4+ T Cells of Patients with Systemic Lupus Erythematosus. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-509125
American Medical Association (AMA)
Zhao, Ming& Tang, Jinling& Gao, Fei& Wu, Xiaoyan& Liang, Yunsheng& Yin, Heng…[et al.]. Hypomethylation of IL10 and IL13 Promoters in CD4+ T Cells of Patients with Systemic Lupus Erythematosus. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-509125
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509125