Specific Dioscorea Phytoextracts Enhance Potency of TCL-Loaded DC-Based Cancer Vaccines
Joint Authors
Lai, Phoency
Yin, Shu-Yi
Chang, Wei-Ting
Wen, Chih-Chun
Chen, Hui-Ming
Wei, Wen-Chi
Wang, Cheng-Hsin
Chen, Yung-Hsiang
Yang, Ning-Sun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-07
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Dioscorea tuber phytoextracts can confer immunomodulatory activities ex vivo and improve regeneration of bone marrow cells in vivo.
In present study, we evaluated specific Dioscorea phytoextracts for use ex vivo as a bone-marrow-derived dendritic cell- (DC-) based vaccine adjuvant for cancer immunotherapy.
Fractionated Dioscorea extracts (DsII) were assayed for their effect on maturation and functions of DC ex vivo and antimelanoma activity of DC-based vaccine in vivo.
The phytoextract from 50–75% ethanol-precipitated fraction of Dioscorea alata var.
purpurea Tainung no.
5 tuber, designated as DsII-TN5, showed a strong augmentation of tumor cell lysate- (TCL-) loaded DC-mediated activation of T-cell proliferation.
DsII-TN5 stimulated the expression of CD40, CD80, CD86, and IL-1β in TCL-loaded DCs and downregulated the expression of TGF-β1.
DC vaccines prepared by a specific schema (TCL (2 h) + LPS (22 h)) showed the strongest antitumor activity.
DsII-TN5 as a DC vaccine adjuvant showed strong antimelanoma activity and reduced myeloid-derived suppressor cell (MDSC) population in tested mice.
DsII-TN5 can also activate DCs to enhance Th1- and Th17-related cytokine expressions.
Biochemical analysis showed that DsII-TN5 consists mainly of polysaccharides containing a high level (53%) of mannose residues.
We suggest that DsII-TN5 may have potential for future application as a potent, cost-effective adjuvant for DC-based cancer vaccines.
American Psychological Association (APA)
Chang, Wei-Ting& Chen, Hui-Ming& Yin, Shu-Yi& Chen, Yung-Hsiang& Wen, Chih-Chun& Wei, Wen-Chi…[et al.]. 2013. Specific Dioscorea Phytoextracts Enhance Potency of TCL-Loaded DC-Based Cancer Vaccines. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-509222
Modern Language Association (MLA)
Chang, Wei-Ting…[et al.]. Specific Dioscorea Phytoextracts Enhance Potency of TCL-Loaded DC-Based Cancer Vaccines. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-509222
American Medical Association (AMA)
Chang, Wei-Ting& Chen, Hui-Ming& Yin, Shu-Yi& Chen, Yung-Hsiang& Wen, Chih-Chun& Wei, Wen-Chi…[et al.]. Specific Dioscorea Phytoextracts Enhance Potency of TCL-Loaded DC-Based Cancer Vaccines. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-509222
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509222