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Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways : Uroporphyrinogen Decarboxylase and Transketolase
Joint Authors
Pedrajas, José Rafael
Padilla, C. Alicia
Bárcena, José Antonio
McDonagh, Brian
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Uroporphyrinogen decarboxylase (Hem12p) and transketolase (Tkl1p) are key mediators of two critical processes within the cell, heme biosynthesis, and the nonoxidative part of the pentose phosphate pathway (PPP).
The redox properties of both Hem12p and Tkl1p from Saccharomyces cerevisiae were investigated using proteomic techniques (SRM and label-free quantification) and biochemical assays in cell extracts and in vitro with recombinant proteins.
The in vivo analysis revealed an increase in oxidized Cys-peptides in the absence of Grx2p, and also after treatment with H2O2 in the case of Tkl1p, without corresponding changes in total protein, demonstrating a true redox response.
Out of three detectable Cys residues in Hem12p, only the conserved residue Cys52 could be modified by glutathione and efficiently deglutathionylated by Grx2p, suggesting a possible redox control mechanism for heme biosynthesis.
On the other hand, Tkl1p activity was sensitive to thiol redox modification and although Cys622 could be glutathionylated to a limited extent, it was not a natural substrate of Grx2p.
The human orthologues of both enzymes have been involved in certain cancers and possess Cys residues equivalent to those identified as redox sensitive in yeast.
The possible implication for redox regulation in the context of tumour progression is put forward.
American Psychological Association (APA)
McDonagh, Brian& Pedrajas, José Rafael& Padilla, C. Alicia& Bárcena, José Antonio. 2013. Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways : Uroporphyrinogen Decarboxylase and Transketolase. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-509268
Modern Language Association (MLA)
McDonagh, Brian…[et al.]. Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways : Uroporphyrinogen Decarboxylase and Transketolase. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-509268
American Medical Association (AMA)
McDonagh, Brian& Pedrajas, José Rafael& Padilla, C. Alicia& Bárcena, José Antonio. Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways : Uroporphyrinogen Decarboxylase and Transketolase. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-509268
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509268